Extremely high brightness from polymer-encapsulated quantum dots for two-photon cellular and deep-tissue imaging.
Extremely high brightness from polymer-encapsulated quantum dots for two-photon cellular and deep-tissue imaging.
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用于双光子细胞和深层组织成像的聚合物封装量子点的极高亮度
DOI：
10.1038/srep09908
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发表时间：
2015-04-24
影响因子：
4.6
通讯作者：
Jiang Y
中科院分区：
文献类型：
--
作者：
Fan Y;Liu H;Han R;Huang L;Shi H;Sha Y;Jiang Y
具有高双光子吸收（TPA）的材料在诸如三维数据存储、TP显微术（TPM）和光动力学治疗（PDT）等一系列领域中是高度期望的。具体地，对于TPM，高度期望高TP激发（TPE）亮度（σ × λ，其中σ是TPA横截面，λ是荧光量子产率）、优异的光稳定性和最小的细胞毒性。然而，当TPA材料通过分子工程或纳米颗粒配制转移到水介质中时，它们通常遭受量子产率（QY）的严重降低。本文报道了一种简便、有效的制备聚合物封装量子点的方法。有趣的是，QY从最初的0.33（在THF中的QD）显著提高到0.84（在水中的P-QD）。根据荧光衰减寿命分析，QY的显著增强主要来自于有效地阻断表面陷阱态的非辐射衰减途径。P-QD具有极高的亮度（σ × λ高达6.2 × 106 GM）、高的光稳定性、优异的胶体稳定性和最小的细胞毒性。 以P-QD为探针实现了高信噪比（> 100）的高质量细胞TP成像和穿透深度为2200 μm的组织成像。
Materials possessing high two photon absorption (TPA) are highly desirable for a range of fields, such as three-dimensional data storage, TP microscopy (TPM) and photodynamic therapy (PDT). Specifically, for TPM, high TP excitation (TPE) brightness (σ × ϕ, where σ is TPA cross-sections and ϕ is fluorescence quantum yield), excellent photostability and minimal cytotoxicity are highly desirable. However, when TPA materials are transferred to aqueous media through molecule engineering or nanoparticle formulation, they usually suffer from the severely decrease of quantum yield (QY). Here, we report a convenient and efficient method for preparing polymer-encapsulated quantum dots (P-QD). Interestingly, the QY was considerably enhanced from original 0.33 (QDs in THF) to 0.84 (P-QD in water). This dramatic enhancement in QY is mainly from the efficiently blocking nonradiative decay pathway from the surface trap states, according to the fluorescence decay lifetimes analysis. The P-QD exhibits extremely high brightness (σ × ϕ up to 6.2 × 106 GM), high photostability, excellent colloidal stability and minimal cytotoxicity. High quality cellular TP imaging with high signal-to-background ratio (> 100) and tissue imaging with a penetration depth of 2200 μm have been achieved with P-QD as probe.
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影响因子：
4.9
作者：
Jang, E;Jun, S;Pu, L
通讯作者：
Pu, L
影响因子：
4.9
作者：
Kim, S;Pudavar, HE;Prasad, PN
通讯作者：
Prasad, PN
影响因子：
15
作者：
He, Yao;Su, Yuanyuan;Lee, Shuit-Tong
通讯作者：
Lee, Shuit-Tong
影响因子：
17.1
作者：
Hu X;Gao X
通讯作者：
Gao X
影响因子：
15
作者：
Kim, Sehoon;Ohulchanskyy, Tymish Y.;Prasad, Paras N.
通讯作者：
Prasad, Paras N.