Whole-plant growth and N allocation in transgenic rice plants with decreased content of ribulose-1,5-bisphosphate carboxylase under different CO2 partial pressures
Whole-plant growth and N allocation in transgenic rice plants with decreased content of ribulose-1,5-bisphosphate carboxylase under different CO2 partial pressures
复制标题
不同CO2分压下核酮糖-1,5-二磷酸羧化酶含量降低的转基因水稻植株的全株生长和氮素分配
DOI：
10.1071/pp99094
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
N. Yamamoto
中科院分区：
文献类型：
--
作者：
A. Makino;Masayo Harada;K. Kaneko;T. Mae;T. Shimada;N. Yamamoto
研究了Rubisco小亚基反义基因转基因水稻（Oryza sativa L.）在36和100 Pa CO2条件下14 h （1000 mol量子m-2 s-1）的生长情况。利用两系反义植物；一种含有65%野生型Rubisco，另一种含有40%野生型Rubisco。结果表明，在36 Pa CO2条件下生长的反义植物的最终生物量明显低于野生型。然而，在反义植物中发现了几种补偿现象。叶片生物量分配增加，叶片氮投入优先。叶片衰老也被推迟。当二氧化碳浓度升高到100 Pa时，反义植物达到了与野生植物相似的大小。然而，虽然选择具有65%野生型Rubisco的反义植物作为CO2饱和光合作用的最佳Rubisco含量，但其最终生物量并不大于野生型植物。这可能是由于生长早期（21-42 d） rubisco -反义效应较强所致。所选反义植株在d 42后的生长氮利用效率更高。因此，单叶水平氮利用效率的提高并不一定导致全株水平生物量的增加。
Growth of transgenic rice (Oryza sativa L.) with an antisense gene to the small subunit of Rubisco was analysed under 36 and 100 Pa CO2 during a 14-h photoperiod (1000 mol quanta m–2 s–1). Two lines of the antisense plants were used; one with 65% wild-type Rubisco and the other with 40% wild-type Rubisco. The plants were grown hydroponically for 70 d. The final biomass of the antisense plants grown in 36 Pa CO2 was much smaller than that of the wild-type plant. However, several compen-sation phenomena were found in the antisense plants. Increased biomass allocation to leaf blades and preferential N investment in leaf blades were observed. Leaf senescence was also delayed. Elevated CO2 levels up to 100 Pa caused the antisense plants to achieve a size similar to that of the wild-type plant. However, although the antisense plant with 65% wild-type Rubisco was selected as a plant with optimal Rubisco content for CO2 -saturated photosynthesis, its final biomass was not greater than that of the wild-type plant. This may have been caused by a relatively strong Rubisco-antisense effect during the early stage of growth (21–42 d). N-use efficiency for growth after d 42 was greater in the selected antisense plant. Thus, improvement of N-use efficiency at the level of a single leaf did not necessarily lead to greater production of biomass at the whole-plant level.
影响因子：
11.6
作者：
HENSEL, LL;GRBIC, V;BLEECKER, AB
通讯作者：
BLEECKER, AB