Morphological classification of NADPHd-positive and -negative myenteric neurons in the porcine small intestine
Morphological classification of NADPHd-positive and -negative myenteric neurons in the porcine small intestine
复制标题
猪小肠NADPHd阳性和阴性肌间神经元的形态学分类
DOI：
10.1007/s004410050738
复制
发表时间：
1996
影响因子：
3.6
通讯作者：
W. Stach
中科院分区：
文献类型：
--
作者：
A. Brehmer;W. Stach
摘要：在这项研究中，神经元型一氧化氮合酶(NNOS)相关的NADPHd反应与银浸法相结合，显示了猪肠内氮能和非氮能肠神经元的形态。根据颜色组合，可将NADPHd阳性但未染色的神经细胞群、NADPHd阴性但未染色的神经细胞群和NADPHd阳性但未染色的神经细胞群区分开来。前两组神经元均可从形态上进行分类。NADPHd阳性细胞和浸泡细胞呈III型和VI型形态，前者较好地位于小肠上部，后者位于小肠下部。这两个项目在很大程度上都是朝着流产的方向发展。NADPHd阴性但银染的神经元有口腔投射的I型神经元、主要沿周向投射的侧突投射的II型神经元、垂直投射到粘膜下神经丛的IV型神经元和垂直投射到粘膜下神经丛的V型神经元。鉴于NADPHd和nNOS是相同的，我们得出结论：III型和VI型神经元是氮能神经元，而I、II、IV和V型神经元是非氮能神经元。相当数量的较小的NADPHd阳性但未受孕的神经元无法分类。
Abstract.In this study, the neuronal nitric oxide synthase (nNOS)-related NADPHd reaction was combined with a silver impregnation method to visualize the morphology of nitrergic and non-nitrergic enteric neurons in the pig. Based on colour combination, NADPHd-positive and impregnated, NADPHd-negative but impregnated and NADPHd-positive but non-impregnated groups of nerve cells could be distinguished in whole-mounts of the small intestine. Neurons of the first two groups could be classified morphologically. NADPHd-positive and impregnated cells showed type III and type VI morphology, the first being located preferably in the upper, the latter in the lower small intestine. Both project largely in an aboral direction. NADPHd-negative but silver impregnated are the orally projecting type I, the adendritic, mostly circumferentially projecting type II, the vertically – to the submucous plexuses – projecting type IV and the aborally projecting type V neurons. Given that NADPHd and nNOS are identical, we conclude that type III and type VI neurons are nitrergic and type I, II, IV and V neurons are non-nitrergic. A considerable number of mostly smaller NADPHd-positive but non-impregnated neurons could not be classified.
DOI：
10.1073/pnas.88.17.7797
发表时间：
1991-09-01
影响因子：
11.1
作者：
DAWSON, TM;BREDT, DS;SNYDER, SH
通讯作者：
SNYDER, SH