Developing dual herbicide tolerant transgenic rice plants for sustainable weed management.
Developing dual herbicide tolerant transgenic rice plants for sustainable weed management.
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DOI：
10.1038/s41598-018-29554-9
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发表时间：
2018-08-02
影响因子：
4.6
通讯作者：
Reddy MK
中科院分区：
文献类型：
--
作者：
Fartyal D;Agarwal A;James D;Borphukan B;Ram B;Sheri V;Agrawal PK;Achary VMM;Reddy MK
除草剂是现代杂草综合治理体系的重要组成部分。然而，单一除草剂的持续使用导致抗性杂草频繁进化，这进一步给其管理带来挑战。为了克服这种情况，交替使用多种除草剂和传统的杂草管理方法是合适的，并且是推荐的。多种耐除草剂作物的开发仍处于起步阶段，只有少数具有耐除草剂性状的作物被报道并商业化。在这项研究中，我们开发了对苯磺隆 (BM) 和草铵膦除草剂均具有耐受性的转基因水稻植物。水稻 AHAS（乙酰羟酸合酶）的除草剂耐受突变体与密码子优化的细菌 bar 基因一起过表达。开发的转基因品系在植物发育的各个阶段都表现出对两种除草剂的显着耐受性。所选的转基因品系在种子萌发阶段表现出对100μM BM和30mg/L草胺膦的耐受性增加。叶面施用进一步证实了对 300μM BM 和 2% basta 除草剂的双重耐受性，且没有任何显着的生长和产量损失。双重除草剂耐受性转基因植物的开发为现代农业系统中可持续杂草管理的作物除草剂耐受性知识增添了更多信息。
Herbicides are important constituents of modern integrated weed management system. However, the continuous use of a single herbicide leads to the frequent evolution of resistant weeds which further challenges their management. To overcome this situation, alternating use of multiple herbicides along with conventional weed-management practices is suitable and recommended. The development of multiple herbicide-tolerant crops is still in its infancy, and only a few crops with herbicide tolerance traits have been reported and commercialized. In this study, we developed transgenic rice plants that were tolerant to both bensulfuron methyl (BM) and glufosinate herbicides. The herbicide tolerant mutant variant of rice AHAS (Acetohydroxyacid synthase) was overexpressed along with codon optimized bacterial bar gene. The developed transgenic lines showed significant tolerance to both herbicides at various stages of plant development. The selected transgenic lines displayed an increased tolerance against 100 μM BM and 30 mg/L phosphinothricin during seed germination stage. Foliar applications further confirmed the dual tolerance to 300 μM BM and 2% basta herbicides without any significant growth and yield penalties. The development of dual-herbicide-tolerant transgenic plants adds further information to the knowledge of crop herbicide tolerance for sustainable weed management in modern agricultural system.
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影响因子：
4.3
作者：
Piao, Zhongze;Wang, Wei;Fang, Jun
通讯作者：
Fang, Jun
影响因子：
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作者：
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3
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影响因子：
3.5
作者：
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通讯作者：
Bonny, Sylvie