Differential effects of antigens from L. braziliensis isolates from disseminated and cutaneous leishmaniasis on in vitro cytokine production.
Differential effects of antigens from L. braziliensis isolates from disseminated and cutaneous leishmaniasis on in vitro cytokine production.
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DOI：
10.1186/1471-2334-6-75
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发表时间：
2006-04-25
影响因子：
3.7
通讯作者：
Carvalho EM
中科院分区：
文献类型：
--
作者：
Leopoldo PT;Machado PR;Almeida RP;Schriefer A;Giudice A;de Jesus AR;Ho JL;Guimarães LH;Bacellar O;Carvalho EM
播散性利什曼病是一种新兴的传染病，主要由利什曼原虫引起。巴西利什曼病，其具有与皮肤利什曼病不同的临床和组织病理学特征。本研究观察了15例播散性利什曼病和24例皮肤利什曼病患者外周血单个核细胞（PBMC）在体外培养条件下，经L.巴西利什曼病抗原基因分型为播散性利什曼病或皮肤利什曼病分离株。无论L.巴西利什曼原虫抗原，皮肤利什曼病患者PBMC产生的IFN-γ显著高于播散性利什曼病患者PBMC。皮肤利什曼病患者PBMC中TNF-α水平仅在基因型皮肤利什曼病抗原刺激下显著高于播散性利什曼病患者。PBMC产生IL-5和IL-10的水平非常低，并且在来自播散性利什曼病和皮肤利什曼病患者的PBMC中相似。采用两种L.在配对分析中，我们发现，L.巴西利什曼原虫基因分型为播散性利什曼病分离株，比L.巴西利什曼原虫基因型为皮肤利什曼病分离株，在两种疾病中触发IFN-γ和TNF-α产生，而在皮肤利什曼病患者中仅触发IL-5产生。这项研究提供了证据表明，抗原制备的基因型不同菌株的L。braziliensis诱导不同程度的免疫应答。寄生虫和宿主对L.巴西人感染
Disseminated leishmaniasis is an emerging infectious disease, mostly due to L. braziliensis, which has clinical and histopathological features distinct from cutaneous leishmaniasis. In the current study we evaluated the in vitro production of the cytokines IFN-γ, TNF-α, IL-5 and IL-10 by peripheral blood mononuclear cells (PBMC) from 15 disseminated leishmaniasis and 24 cutaneous leishmaniasis patients upon stimulation with L. braziliensis antigens genotyped as disseminated leishmaniasis or cutaneous leishmaniasis isolates. Regardless of the source of L. braziliensis antigens, PBMC from cutaneous leishmaniasis patients produced significantly higher IFN-γ than PBMC from disseminated leishmaniasis patients. Levels of TNF-α by PBMC from cutaneous leishmaniasis patients were significantly higher than disseminated leishmaniasis patients only when stimulated by genotyped cutaneous leishmaniasis antigens. The levels of IL-5 and IL-10 production by PBMC were very low and similar in PBMCs from both disseminated leishmaniasis and cutaneous leishmaniasis patients. The immune response of each patient evaluated by the two L. braziliensis antigens was assessed in a paired analysis in which we showed that L. braziliensis genotyped as disseminated leishmaniasis isolate was more potent than L. braziliensis genotyped as cutaneous leishmaniasis isolate in triggering IFN-γ and TNF-α production in both diseases and IL-5 only in cutaneous leishmaniasis patients. This study provides evidence that antigens prepared from genotypically distinct strains of L. braziliensis induce different degrees of immune response. It also indicates that both parasite and host play a role in the outcome of L. braziliensis infection.
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影响因子：
2.1
作者：
Bomfim, G;Nascimento, C;Barral, A
通讯作者：
Barral, A
影响因子：
4.6
作者：
CACERESDITTMAR, G;TAPIA, FJ;CONVIT, J
通讯作者：
CONVIT, J
影响因子：
2.7
作者：
CARVALHO, EM;BARRAL, A;MARSDEN, P
通讯作者：
MARSDEN, P
影响因子：
6.4
作者：
Bacellar, O;Brodskyn, C;Carvalho, EM
通讯作者：
Carvalho, EM
影响因子：
3.1
作者：
Schriefer, A;Schriefer, ALF;Carvalho, EM
通讯作者：
Carvalho, EM