Memory B cells from a subset of treatment-naïve relapsing-remitting multiple sclerosis patients elicit CD4(+) T-cell proliferation and IFN-γ production in response to myelin basic protein and myelin oligodendrocyte glycoprotein.
Memory B cells from a subset of treatment-naïve relapsing-remitting multiple sclerosis patients elicit CD4(+) T-cell proliferation and IFN-γ production in response to myelin basic protein and myelin oligodendrocyte glycoprotein.
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DOI：
10.1002/eji.201040516
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发表时间：
2010-10
影响因子：
5.4
通讯作者：
Monson, Nancy L.
中科院分区：
文献类型：
--
作者：
Harp, Christopher T.;Ireland, Sara;Davis, Laurie S.;Remington, Gina;Cassidy, Bonnie;Cravens, Petra D.;Stuve, Olaf;Lovett-Racke, Amy E.;Eagar, Todd N.;Greenberg, Benjamin M.;Racke, Michael K.;Cowell, Lindsay G.;Karandikar, Nitin J.;Frohman, Elliot M.;Monson, Nancy L.
最近的证据表明，B 细胞和 T 细胞的相互作用可能在复发缓解型多发性硬化症 (RRMS) 疾病发病机制中发挥着至关重要的作用。我们假设，来自 RRMS 患者的记忆 B 细胞库可能特异地含有一组有效的神经抗原呈递细胞，支持神经抗原反应性 T 细胞增殖和细胞因子分泌。为了检验这一假设，我们比较了 RRMS 患者的记忆 B 细胞与 RRMS 患者的初始 B 细胞以及健康供体 (HD) 的记忆 B 细胞和初始 B 细胞的 CD80 和 HLA-DR 表达、IL-10 和 LTα 分泌、神经抗原结合能力以及神经抗原呈递。我们从一些 RRMS 患者中鉴定出记忆 B 细胞，它们响应髓磷脂碱性蛋白 (MBP) 和髓磷脂少突胶质细胞糖蛋白 (MOG) 的反应，引发 CD4+ T 细胞增殖和 IFN-γ 分泌。尽管RRMS和HD记忆B细胞之间促进有效抗原呈递的表型参数相似，但在HD记忆B细胞中没有观察到响应MBP和MOG而引发CD4+T细胞增殖的相应能力。我们的结果首次证明，RRMS 中的记忆 B 细胞库含有可以激活 T 细胞的神经抗原特异性 B 细胞。
Recent evidence suggests that B and T cell interactions may be paramount in relapsing remitting multiple sclerosis (RRMS) disease pathogenesis. We hypothesized that memory B cell pools from RRMS patients may specifically harbor a subset of potent neuro-antigen presenting cells that support neuro-antigen reactive T cell proliferation and cytokine secretion. To test this hypothesis, we compared CD80 and HLA-DR expression, IL-10 and LTα secretion, neuro-antigen binding capacity, and neuro-antigen presentation by memory B cells from RRMS patients to naïve B cells from RRMS patients and to memory and naïve B cells from healthy donors (HD). We identified memory B cells from some RRMS patients that elicited CD4+ T cell proliferation and IFN-γ secretion in response to myelin basic protein (MBP) and myelin oligodendrocyte glycoprotein (MOG). Notwithstanding the fact that the phenotypic parameters that promote efficient antigen presentation were observed to be similar between RRMS and HD memory B cells, a corresponding capability to elicit CD4+ T cell proliferation in response to MBP and MOG was not observed in HD memory B cells. Our results demonstrate for the first time that the memory B cell pool in RRMS harbors neuro-antigen specific B cells that can activate T cells.
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影响因子：
11.2
作者：
Correale, Jorge;Villa, Andres
通讯作者：
Villa, Andres
影响因子：
3.1
作者：
Corinti, S;Medaglini, D;Girolomoni, G
通讯作者：
Girolomoni, G
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15.9
作者：
Genc, K;Dona, DL;Reder, AT
通讯作者：
Reder, AT
影响因子：
15.9
作者：
DEROSBO, NK;MILO, R;BENNUN, A
通讯作者：
BENNUN, A
影响因子：
15.9
作者：
Cappione, A;Anolik, JH;Sanz, I
通讯作者：
Sanz, I