Quartz crystal microbalance-based biosensor for the detection of α-thalassemia 1 (SEA deletion)
Quartz crystal microbalance-based biosensor for the detection of α-thalassemia 1 (SEA deletion)
复制标题
基于石英晶体微天平的生物传感器，用于检测 α-地中海贫血 1（SEA 缺失）
DOI：
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发表时间：
2010
影响因子：
6.8
通讯作者：
W. Ittarat
中科院分区：
文献类型：
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作者：
Sirinart Chomean;Tiparat Potipitak;C. Promptmas;W. Ittarat
摘要背景：DNA压电生物传感器由于不需要任何标记或染色而成为分子医学中很有前途的工具。本研究建立了基于石英晶体微天平（QCM）的DNA压电生物传感器，用于鉴定导致α-地中海贫血1 （SEA缺失）的异常基因。方法：在石英晶体的功能化金电极上包覆亲和素，并连接生物素化DNA探针。扩增α-地中海贫血1的靶基因并与固定化探针杂交。石英共振频率的变化表明DNA杂交。新的α-地中海贫血1生物传感器的诊断能力通过已知和未知的血液样本进行验证。用β-地中海贫血和α-地中海贫血2的样本检测特异性。并对传感器的稳定性进行了评价。结果：该生物传感器能够从正常基因型中清晰地识别α-地中海贫血1 （SEA缺失）的携带者和疾病状态。鉴定精度与标准凝胶电泳一致。由于未发现与β-地中海贫血和α-地中海贫血2的交叉反应，因此仅对α-地中海贫血1具有特异性。该传感器可在室温下保存长达6个月，识别精度保持一致。结论：基于无标记QCM的生物传感器可用于诊断α-地中海贫血1 （SEA缺失）的异常人珠蛋白基因。其准确度、特异度和灵敏度与标准方法相当。其稳定的诊断效能长达6个月，预示着其在地中海贫血控制规划中的实际应用。中华临床医学杂志，2010;48(4):1245 - 1245。
Abstract Background: DNA piezoelectric biosensors have become a promising tool in molecular medicine since they do not require any label or staining. Here, a DNA piezoelectric biosensor based on a quartz crystal microbalance (QCM) was created to identify abnormal genes causing α-thalassemia 1 (SEA deletion). Methods: The functionalized gold electrode of the quartz crystal was coated with avidin and the biotinylated DNA probe was attached. The target gene causing α-thalassemia 1 was amplified and hybridized with the immobilized probe. DNA hybridization was indicated by changes in the quartz resonance frequencies. Diagnostic ability of the new α-thalassemia 1 biosensor was validated using both known and unknown blood samples. Specificity was tested using samples of β-thalassemia and α-thalassemia 2. Stability of the sensor was also evaluated. Results: The new biosensor could clearly identify α-thalassemia 1 (SEA deletion), both carrier and disease states, from the normal genotype. Identification accuracy was compatible to the standard gel electrophoresis. It was specific only to α-thalassemia 1 since no cross reaction was found with β-thalassemia and α-thalassemia 2. The sensor could be kept at room temperature up to 6 months with consistent identification accuracy. Conclusions: The label free QCM based biosensor was successfully developed to diagnose an abnormal human globin gene causing α-thalassemia 1 (SEA deletion). Its accuracy, specificity and sensitivity were comparable to the standard method. Its stable diagnostic potency up to 6 months implied its field application in thalassemic control program. Clin Chem Lab Med 2010;48:1247–54.
DOI：
10.1007/978-1-60327-418-0_1
发表时间：
2010
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
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作者：
Liu, Yang;Wilson, W David
通讯作者：
Wilson, W David