Rapid change of fecal microbiome and disappearance of Clostridium difficile in a colonized infant after transition from breast milk to cow milk.
Rapid change of fecal microbiome and disappearance of Clostridium difficile in a colonized infant after transition from breast milk to cow milk.
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DOI：
10.1186/s40168-016-0198-6
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发表时间：
2016-10-07
期刊：
影响因子：
15.5
通讯作者：
Boone JH
中科院分区：
文献类型：
--
作者：
Davis MY;Zhang H;Brannan LE;Carman RJ;Boone JH
艰难梭菌是最常见的已知原因与腹泻相关。在抗生素干扰肠道菌群的情况下，C.艰难梭菌建立生长并释放毒素A和B，其引起宿主的组织损伤。C.艰难梭菌感染疾病的范围从轻度腹泻到伪膜性结肠炎和中毒性巨结肠。有趣的是，10- 50%的婴儿是C的无症状携带者.很难本研究对C.艰难梭菌在婴儿中的定植揭示了C.在肠道微生物群中的艰难存在。50份粪便样本，每周从一名5.5至17个月大的无症状C. 16 S rRNA基因测序分析。转基因和非转基因C.艰难梭菌菌株以及超过100，000倍波动的C.观察艰难梭菌计数。C.在测试期间，在一些婴儿粪便样本中检测到艰难梭菌毒素，但婴儿从未腹泻。尽管母乳喂养期间粪便微生物群是稳定的，但在从母乳过渡到牛奶的5天内观察到微生物群组成的显著和永久变化。C. difficile与12.5个月时断奶一致。拟杆菌属的相对丰度增加，布劳特氏菌属，副拟杆菌属，粪球菌属，瘤胃球菌属，和颤螺旋菌属（Oscillospira spp.）以及双歧杆菌属的减少，乳杆菌属，埃希氏菌属，和梭菌属（Clostridium spp.）在断奶期间观察。微生物组组成的变化伴随着粪便pH值从5.5逐渐增加到7。在婴儿早期数量较少的细菌群，在断奶后相对数量增加，可能是导致C。很难 本文的在线版本（doi：10.1186/s40168-016-0198-6）包含补充材料，可供授权用户使用。
Clostridium difficile is the most common known cause of antibiotic-associated diarrhea. Upon the disturbance of gut microbiota by antibiotics, C. difficile establishes growth and releases toxins A and B, which cause tissue damage in the host. The symptoms of C. difficile infection disease range from mild diarrhea to pseudomembranous colitis and toxic megacolon. Interestingly, 10–50 % of infants are asymptomatic carriers of C. difficile. This longitudinal study of the C. difficile colonization in an infant revealed the dynamics of C. difficile presence in gut microbiota. Fifty fecal samples, collected weekly between 5.5 and 17 months of age from a female infant who was an asymptomatic carrier of C. difficile, were analyzed by 16S rRNA gene sequencing. Colonization switching between toxigenic and non-toxigenic C. difficile strains as well as more than 100,000-fold fluctuations of C. difficile counts were observed. C. difficile toxins were detected during the testing period in some infant stool samples, but the infant never had diarrhea. Although fecal microbiota was stable during breast feeding, a dramatic and permanent change of microbiota composition was observed within 5 days of the transition from human milk to cow milk. A rapid decline and eventual disappearance of C. difficile coincided with weaning at 12.5 months. An increase in the relative abundance of Bacteroides spp., Blautia spp., Parabacteroides spp., Coprococcus spp., Ruminococcus spp., and Oscillospira spp. and a decrease of Bifidobacterium spp., Lactobacillus spp., Escherichia spp., and Clostridium spp. were observed during weaning. The change in microbiome composition was accompanied by a gradual increase of fecal pH from 5.5 to 7. The bacterial groups that are less abundant in early infancy, and that increase in relative abundance after weaning, likely are responsible for the expulsion of C. difficile. The online version of this article (doi:10.1186/s40168-016-0198-6) contains supplementary material, which is available to authorized users.
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影响因子：
17.1
作者：
Ardeshir A;Narayan NR;Méndez-Lagares G;Lu D;Rauch M;Huang Y;Van Rompay KK;Lynch SV;Hartigan-O'Connor DJ
通讯作者：
Hartigan-O'Connor DJ
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.7
作者：
Giel JL;Sorg JA;Sonenshein AL;Zhu J
通讯作者：
Zhu J
影响因子：
4.4
作者：
Karav, Sercan;Le Parc, Annabelle;Mills, David A.
通讯作者：
Mills, David A.
影响因子：
3.2
作者：
Brigham, Christopher;Caughlan, Ruth;Malamy, Michael H.
通讯作者：
Malamy, Michael H.