Motif mismatches in microsatellites: insights from genome-wide investigation among 20 insect species.
Motif mismatches in microsatellites: insights from genome-wide investigation among 20 insect species.
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DOI：
10.1093/dnares/dsu036
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发表时间：
2015-02
期刊：
影响因子：
--
通讯作者：
Severson DW
中科院分区：
文献类型：
--
作者：
Behura SK;Severson DW
我们对代表5个分类目的20种昆虫的微卫星基序错配进行了详细的全基因组比较研究。结果表明，在这些物种中发现的不同比例（~ 15-46%）的微卫星在基序结构上不完美，并且它们在基因组内的染色体分布也不同。结果表明，不完全重复序列的基因组丰度与微卫星基序错配的长度和数量显著相关。此外，错配数量较多的微卫星在基因组中的丰度往往较低，这表明重复基序的序列异质性是微卫星基因组丰度的关键决定因素。这种关系似乎是微卫星的普遍特征，甚至在不相关的物种，如酵母、蛔虫、老鼠和人类中也是如此。我们通过研究微卫星位点内发现的基序错配模式和单核苷酸多态性的等位基因序列，为基序异质性和微卫星基因组丰度之间的进化联系提供了机制解释。利用果蝇参考遗传小组的数据，我们进一步表明等位基因变异模式调节了微卫星的基序异质性，并提供了蛋白质编码基因中发现的特定不完美微卫星的等位基因年龄的估计。
We present a detailed genome-wide comparative study of motif mismatches of microsatellites among 20 insect species representing five taxonomic orders. The results show that varying proportions (∼15–46%) of microsatellites identified in these species are imperfect in motif structure, and that they also vary in chromosomal distribution within genomes. It was observed that the genomic abundance of imperfect repeats is significantly associated with the length and number of motif mismatches of microsatellites. Furthermore, microsatellites with a higher number of mismatches tend to have lower abundance in the genome, suggesting that sequence heterogeneity of repeat motifs is a key determinant of genomic abundance of microsatellites. This relationship seems to be a general feature of microsatellites even in unrelated species such as yeast, roundworm, mouse and human. We provide a mechanistic explanation of the evolutionary link between motif heterogeneity and genomic abundance of microsatellites by examining the patterns of motif mismatches and allele sequences of single-nucleotide polymorphisms identified within microsatellite loci. Using Drosophila Reference Genetic Panel data, we further show that pattern of allelic variation modulates motif heterogeneity of microsatellites, and provide estimates of allele age of specific imperfect microsatellites found within protein-coding genes.
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影响因子：
5.8
作者：
Kofler, Robert;Schloetterer, Christian;Lelley, Tamas
通讯作者：
Lelley, Tamas
影响因子：
5.3
作者：
Coelho, M;Luiselli, D;Rocha, J
通讯作者：
Rocha, J
影响因子：
3.5
作者：
Bacon, AL;Farrington, SM;Dunlop, MG
通讯作者：
Dunlop, MG
影响因子：
3.9
作者：
Britt-Compton, B.;Baird, D. M.
通讯作者：
Baird, D. M.
DOI：
10.1073/pnas.91.8.3166
发表时间：
1994-04-12
影响因子：
11.1
作者：
DIRIENZO, A;PETERSON, AC;FREIMER, NB
通讯作者：
FREIMER, NB