Recoverin regulates light-dependent phosphodiesterase activity in retinal rods.
Recoverin regulates light-dependent phosphodiesterase activity in retinal rods.
复制标题
DOI：
10.1085/jgp.200308994
复制
发表时间：
2004-06
期刊：
影响因子：
--
通讯作者：
Baylor DA
中科院分区：
文献类型：
--
作者：
Makino CL;Dodd RL;Chen J;Burns ME;Roca A;Simon MI;Baylor DA
Ca2+结合蛋白恢复可能调节视杆和视锥的视觉转导，但其功能作用和作用机制仍有争议。我们比较了对照小鼠和敲除恢复基因的小鼠的视杆细胞的光反应。我们的分析表明，Ca2+恢复延长了适应黑暗的闪光反应，并增加了杆对昏暗稳定光的敏感性。敲除棒具有更快的Ca2+动态，表明恢复蛋白是外段重要的Ca2+缓冲液，但外源缓冲液的掺入并没有恢复野生型行为。我们推断Ca2+恢复蛋白可能通过有效延长光激发视紫质的催化活性来增强光触发磷酸二酯酶的活性。
The Ca2+-binding protein recoverin may regulate visual transduction in retinal rods and cones, but its functional role and mechanism of action remain controversial. We compared the photoresponses of rods from control mice and from mice in which the recoverin gene was knocked out. Our analysis indicates that Ca2+-recoverin prolongs the dark-adapted flash response and increases the rod's sensitivity to dim steady light. Knockout rods had faster Ca2+ dynamics, indicating that recoverin is a significant Ca2+ buffer in the outer segment, but incorporation of exogenous buffer did not restore wild-type behavior. We infer that Ca2+-recoverin potentiates light-triggered phosphodiesterase activity, probably by effectively prolonging the catalytic activity of photoexcited rhodopsin.
登录
查看更多内容
DOI：
10.1073/pnas.250478897
发表时间：
2000-12-05
影响因子：
11.1
作者：
Calvert, PD;Krasnoperova, NV;Lem, J
通讯作者：
Lem, J
影响因子：
16.2
作者：
GRAYKELLER, MP;POLANS, AS;DETWILER, PB
通讯作者：
DETWILER, PB
影响因子：
64.8
作者：
KAWAMURA, S
通讯作者：
KAWAMURA, S
影响因子：
64.8
作者：
KAWAMURA, S;MURAKAMI, M
通讯作者：
MURAKAMI, M
影响因子：
2.5
作者：
BASTIANELLI, E;POLANS, AS;POCHET, R
通讯作者：
POCHET, R