Preservation of bone mass and structure in hibernating black bears (Ursus americanus) through elevated expression of anabolic genes.
Preservation of bone mass and structure in hibernating black bears (Ursus americanus) through elevated expression of anabolic genes.
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通过合成代谢基因的表达升高，在冬眠的黑熊（URSUS Americanus）中保存骨骼质量和结构。
DOI：
10.1007/s10142-012-0266-3
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发表时间：
2012-06
影响因子：
2.9
通讯作者：
Barnes, Brian M.
中科院分区：
文献类型：
--
作者：
Fedorov, Vadim B.;Goropashnaya, Anna V.;Toien, Oivind;Stewart, Nathan C.;Chang, Celia;Wang, Haifang;Yan, Jun;Showe, Louise C.;Showe, Michael K.;Donahue, Seth W.;Barnes, Brian M.
身体活动减少了骨骼上的机械负荷，这导致非冬眠哺乳动物物种骨量和强度的损失。虽然熊在冬眠期间基本上不活动，但它们的骨量和力量没有损失。为了深入了解防止废用性骨丢失的分子机制，我们使用定制的12，800探针cDNA微阵列对冬季冬眠和夏季非冬眠黑熊进行了大规模的骨小梁转录变化筛选。结果表明，在冬季和夏季，熊髂骨中共有241个基因表达差异显著（P <<0.01 and fold change >1.4）。基因本体论和基因集富集分析表明，在冬眠的熊中，六组功能基因中的过表达基因的比例升高，这些基因参与组织形态发生和发育的合成代谢过程，包括骨骼发育、软骨发育和骨生物合成。细胞凋亡基因表现出在冬眠过程中下调的趋势。没有协调的方向变化，检测到参与骨吸收的基因，虽然一些基因负责破骨细胞的形成和分化（Ostf 1，Rab 9a，和c-Fos）显着低表达冬眠熊的骨。多个合成代谢基因的表达升高，而不诱导骨吸收基因，以及骨化相关基因的下调，可能有助于通过冬眠期间长时间的不动来保持骨量和结构的适应性机制。
Physical inactivity reduces mechanical load on the skeleton, which leads to losses of bone mass and strength in non-hibernating mammalian species. Although bears are largely inactive during hibernation, they show no loss in bone mass and strength. To obtain insight into molecular mechanisms preventing disuse bone loss, we conducted a large-scale screen of transcriptional changes in trabecular bone comparing winter hibernating and summer non-hibernating black bears using a custom 12,800 probe cDNA microarray. A total of 241 genes were differentially expressed (P<0.01 and fold change >1.4) in the ilium bone of bears between winter and summer. The Gene Ontology and Gene Set Enrichment Analysis showed an elevated proportion in hibernating bears of overexpressed genes in six functional sets of genes involved in anabolic processes of tissue morphogenesis and development including skeletal development, cartilage development, and bone biosynthesis. Apoptosis genes demonstrated a tendency for downregulation during hibernation. No coordinated directional changes were detected for genes involved in bone resorption, although some genes responsible for osteoclast formation and differentiation (Ostf1, Rab9a, and c-Fos) were significantly underexpressed in bone of hibernating bears. Elevated expression of multiple anabolic genes without induction of bone resorption genes, and the down regulation of apoptosis-related genes, likely contribute to the adaptive mechanism that preserves bone mass and structure through prolonged periods of immobility during hibernation.
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影响因子：
2.8
作者：
Donahue, SW;Galley, SA;McGee, ME
通讯作者：
McGee, ME
影响因子：
4.1
作者：
Kaneps, AJ;Stover, SM;Lane, NE
通讯作者：
Lane, NE
影响因子：
56.9
作者：
Toien, Oivind;Blake, John;Barnes, Brian M.
通讯作者：
Barnes, Brian M.
影响因子：
1.2
作者：
Fink, Trine;Rasmussen, Jeppe G.;Frobert, Ole
通讯作者：
Frobert, Ole
影响因子：
4.1
作者：
Mcgee, Meghan E.;Maki, Aaron J.;Donahue, Seth W.
通讯作者：
Donahue, Seth W.