Activation of ribosomal S6 kinase (RSK) during porcine oocyte maturation
Activation of ribosomal S6 kinase (RSK) during porcine oocyte maturation
复制标题
猪卵母细胞成熟过程中核糖体 S6 激酶 (RSK) 的激活
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
H. Tojo
中科院分区：
文献类型：
--
作者：
K. Sugiura;K. Naito;N. Iwamori;H. Kagii;S. Goto;S. Ohashi;H. Naruoka;E. Yada;K. Yamanouchi;H. Tojo
对猪卵母细胞减数分裂成熟过程中核糖体S6激酶(RSK)的正常动力学进行了研究。用Western blotting方法检测成熟猪卵母细胞中主要的丝裂原活化蛋白(MAP)激酶--RSK和细胞外信号调节蛋白(ERK)的磷酸化状态。用含有S6蛋白的主要磷酸化位点的特定底物多肽测定S6蛋白的活性。RSK的完全磷酸化与ERK的磷酸化相关，并在生发泡破裂之前观察到。无论是新鲜分离的卵母细胞还是培养20h的卵母细胞，S6蛋白的活性都很低。成熟卵母细胞中S6蛋白的活性显著升高，约为新鲜分离卵母细胞的6倍。此外，当MAP激酶特异性抑制剂U0126处理卵母细胞时，RSK的完全磷酸化被抑制，并呈剂量依赖关系，表明RSK是MAP激酶的底物之一。这些结果表明，RSK的激活参与了猪卵母细胞减数分裂成熟的调控。
The normal kinetics of ribosomal S6 kinase (RSK) during the meiotic maturation of porcine oocytes were examined. The phosphorylation states of RSK and extracellular signal-regulated kinase (ERK), major mitogen-activated protein (MAP) kinases in maturating porcine oocytes, were detected by Western blotting analysis. The S6 protein kinase activity was assayed using a specific substrate peptide which contained the major phosphorylation sites of S6 kinase. Full phosphorylation of RSK was correlated with ERK phosphorylation and was observed before germinal vesicle breakdown. S6 kinase activity was low in both freshly isolated and 20 h cultured oocytes. S6 kinase activity was significantly elevated in matured oocytes to a level about 6 times higher than that in freshly isolated oocytes. Furthermore, full phosphorylation of RSK was inhibited when oocytes were treated with U0126, a specific MAP kinase kinase inhibitor, in dose-dependent manner, indicating that RSK is one of the substrates of MAP kinase. These results suggest that the activation of RSK is involved in the regulation of meiotic maturation of porcine oocytes.
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DOI：
10.1016/s0021-9258(17)42478-1
发表时间：
1986-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
E. Erikson;J. Maller
通讯作者：
E. Erikson;J. Maller
DOI：
10.1073/pnas.82.3.742
发表时间：
1985-01-01
影响因子：
11.1
作者：
ERIKSON, E;MALLER, JL
通讯作者：
MALLER, JL
非洲爪蟾卵母细胞成熟过程中核糖体蛋白 S6 激酶的体内磷酸化和激活。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Erikson,E;Maller,JL
通讯作者：
Maller,JL
影响因子：
56.9
作者：
Bhatt, RR;Ferrell, JE
通讯作者：
Ferrell, JE
影响因子：
56.9
作者：
Gross, SD;Schwab, MS;Maller, JL
通讯作者：
Maller, JL