SEDFIT-MSTAR: molecular weight and molecular weight distribution analysis of polymers by sedimentation equilibrium in the ultracentrifuge.
SEDFIT-MSTAR: molecular weight and molecular weight distribution analysis of polymers by sedimentation equilibrium in the ultracentrifuge.
复制标题
DOI：
10.1039/c3an01507f
复制
发表时间：
2014-01-07
期刊：
影响因子：
--
通讯作者：
Harding SE
中科院分区：
文献类型：
--
作者：
Schuck P;Gillis RB;Besong TM;Almutairi F;Adams GG;Rowe AJ;Harding SE
沉降平衡（分析超离心）是测定聚合物平均分子量和分子量分布的最合适的方法之一，因为它具有绝对基础（无构象假设）和固有的分馏能力（不需要柱或膜以及相关的惰性假设）。使用现代仪器，还可以在单次运行中同时运行多达21个样品。它的应用受到严重阻碍，因为在基线确定（包括在空气/溶液弯月面的浓度估计）和分析程序的复杂性方面的困难。我们描述了一种基于智能平滑原理的基线确定新方法，该方法内置于非常受欢迎的平台SEDFIT中，用于分析天然和合成聚合物材料的沉降行为。SEDFIT-MSTAR程序-只需几分钟即可执行-使用四个合成数据集进行测试（包括一个明显非理想的系统）一种天然存在的蛋白质（人IgG 1）和两种天然存在的碳水化合物聚合物（普鲁兰多糖和λ-卡拉胶）在（i）样品中物种的整个分布的重均分子量（ii）“点”的变化方面的差异平均分子量与超浓差电池中的局部浓度和（iii）分子量分布。
Sedimentation equilibrium (analytical ultracentrifugation) is one of the most inherently suitable methods for the determination of average molecular weights and molecular weight distributions of polymers, because of its absolute basis (no conformation assumptions) and inherent fractionation ability (without the need for columns or membranes and associated assumptions over inertness). With modern instrumentation it is also possible to run up to 21 samples simultaneously in a single run. Its application has been severely hampered because of difficulties in terms of baseline determination (incorporating estimation of the concentration at the air/solution meniscus) and complexity of the analysis procedures. We describe a new method for baseline determination based on a smart-smoothing principle and built into the highly popular platform SEDFIT for the analysis of the sedimentation behavior of natural and synthetic polymer materials. The SEDFIT-MSTAR procedure – which takes only a few minutes to perform - is tested with four synthetic data sets (including a significantly non-ideal system) a naturally occurring protein (human IgG1) and two naturally occurring carbohydrate polymers (pullulan and λ–carrageenan) in terms of (i) weight average molecular weight for the whole distribution of species in the sample (ii) the variation in “point” average molecular weight with local concentration in the ultracentrifuge cell and (iii) molecular weight distribution.
登录
查看更多内容
影响因子：
2
作者：
Colfen, H;Harding, SE
通讯作者：
Harding, SE
影响因子：
6.3
作者：
PROVENCHER, SW
通讯作者：
PROVENCHER, SW
影响因子：
3.4
作者：
Schuck, P
通讯作者：
Schuck, P
影响因子：
11.2
作者：
KAWAHARA, K;OHTA, K;NAKAMURA, S
通讯作者：
NAKAMURA, S
影响因子：
6.3
作者：
PROVENCHER, SW
通讯作者：
PROVENCHER, SW