Protective Effect of Baicalin Against TLR4-mediated UVA-induced Skin Inflammation.
Protective Effect of Baicalin Against TLR4-mediated UVA-induced Skin Inflammation.
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DOI：
10.1111/php.13021
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发表时间：
2019-03
影响因子：
3.3
通讯作者：
Yusuf N
中科院分区：
文献类型：
--
作者：
Sherwani MA;Yang K;Jani A;Abed RA;Taufique AK;Dosunmu TG;Yusuf N
众所周知，UVA辐射通过产生活性氧(ROS)和激活炎症过程而导致光老化。此前，我们已经证明了黄芩苷，一种具有抗氧化和抗炎活性的植物来源的黄酮类化合物，可以保护小鼠角质形成细胞免受UVB辐射的损伤。然而，黄芩苷在体内的作用还没有得到很好的研究，特别是在长波紫外线照射的情况下。为探讨黄芩苷对小鼠中波紫外线(UVA)照射后的保护作用及其机制，将小鼠分为急性剂量UVA照射和慢性剂量UVA照射(含或不含黄芩苷或赋形剂)。采集皮肤样本进行组织学染色、RNA提取、流式细胞术和蛋白质提取。我们的结果证明了黄芩苷对UVA诱导的小鼠皮肤氧化损伤和炎症的保护作用。这些效应可能是通过TLR4途径介导的，TLR4通路可能是光化学预防皮肤炎症的靶点。黄芩苷通过上调IL-12和IL-23细胞因子对UVA致小鼠皮肤氧化损伤和炎症的保护作用这些作用可能是通过抑制TLR4途径实现的，而TLR4通路可能是黄芩苷预防皮肤炎症的靶点。
UVA irradiation is known to cause photoaging via production of reactive oxygen species (ROS) and activation of inflammatory processes. Previously, we have demonstrated that baicalin, a plant-derived flavonoid possessing both antioxidant and anti-inflammatory activity, protects mouse keratinocytes against damage from UVB irradiation. However, the role of baicalin in vivo has not been well studied, particularly in the setting of UVA irradiation. To explore the protective effects and mechanisms of baicalin treatment in mice after UVA irradiation, mice were exposed to acute and chronic doses of UVA irradiation with or without baicalin or vehicle. Skin samples were collected for histological staining, RNA isolation, flow cytometry, and protein extraction. Our results demonstrate the protective effect of baicalin against UVA-induced oxidative damage and inflammation in mouse skin. These effects are likely mediated via the TLR4 pathway, which may serve as a target for photochemoprevention against skin inflammation. The protective effect of baicalin treatment against UVA-induced oxidative damage and inflammation in mice skin via upregulation of IL-12 and IL-23 cytokines. These effects are likely mediated via inhibition of TLR4 pathway, which may serve as a target for prevention against skin inflammation by baicalin.
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DOI：
10.1084/jem.20071966
发表时间：
2007-12-24
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Bursch LS;Wang L;Igyarto B;Kissenpfennig A;Malissen B;Kaplan DH;Hogquist KA
通讯作者：
Hogquist KA
影响因子：
3.2
作者：
Harberts E;Fishelevich R;Liu J;Atamas SP;Gaspari AA
通讯作者：
Gaspari AA
影响因子：
5.1
作者：
Lakhan, R.;Baylink, D. J.;Qin, X.
通讯作者：
Qin, X.
影响因子：
4.6
作者：
Ujfaludi, Zsuzsanna;Tuzesi, Agota;Boros, Imre M.
通讯作者：
Boros, Imre M.
影响因子：
5.6
作者：
D'Orazio J;Jarrett S;Amaro-Ortiz A;Scott T
通讯作者：
Scott T