Rapid adaptive optical recovery of optimal resolution over large volumes.
Rapid adaptive optical recovery of optimal resolution over large volumes.
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DOI:
10.1038/nmeth.2925
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发表时间:
2014-06
期刊:
影响因子:
48
通讯作者:
Betzig, Eric
中科院分区:
文献类型:
--
作者:
Wang, Kai;Milkie, Daniel E.;Saxena, Ankur;Engerer, Peter;Misgeld, Thomas;Bronner, Marianne E.;Mumm, Jeff;Betzig, Eric
Using a de-scanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture and a 14 ms update rate. This permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens, and recover diffraction-limited imaging over large (> 240 μm) volumes. We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.
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