Femtosecond Laser Fabricated Spike Structures for Selective Control of Cellular Behavior
Femtosecond Laser Fabricated Spike Structures for Selective Control of Cellular Behavior
复制标题
飞秒激光制造的尖峰结构用于选择性控制细胞行为
DOI：
10.1177/0885328209345553
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发表时间：
2010
影响因子：
2.9
通讯作者：
B. Chichkov
中科院分区：
文献类型：
--
作者：
S. Schlie;É. Fadeeva;J. Koch;A. Ngezahayo;B. Chichkov
在这项研究中，我们研究了飞秒激光产生的微米大小的尖峰结构作为选择性细胞控制的功能表面的潜力。通过改变工艺参数可以轻松调整尖峰尺寸以及平均尖峰到尖峰距离。此外，还可以在硅弹性体等软材料中产生负复制。这允许定制尖峰结构及其代表减少表面接触面积的负复制品的润湿特性。此外，我们研究了材料对细胞行为的影响。通过比较人成纤维细胞和 SH-SY5Y 神经母细胞瘤细胞，我们发现材料的影响是细胞特异性的。细胞不仅形态发生改变，细胞生长也受到影响。然而，神经母细胞瘤细胞在尖峰结构上的增殖速度与在对照表面上的增殖速度相同，而成纤维细胞的增殖则被尖峰结构减少。这些效应可能是由细胞特异性粘附模式引起的，如本工作所示。这些发现表明设计明确的表面微观结构的可能性，它可以以细胞特定的方式控制细胞行为。
In this study we investigate the potential of femtosecond laser generated micrometer sized spike structures as functional surfaces for selective cell controlling. The spike dimensions as well as the average spike to spike distance can be easily tuned by varying the process parameters. Moreover, negative replications in soft materials such as silicone elastomer can be produced. This allows tailoring of wetting properties of the spike structures and their negative replicas representing a reduced surface contact area. Furthermore, we investigated material effects on cellular behavior. By comparing human fibroblasts and SH-SY5Y neuroblastoma cells we found that the influence of the material was cell specific. The cells not only changed their morphology, but also the cell growth was affected. Whereas, neuroblastoma cells proliferated at the same rate on the spike structures as on the control surfaces, the proliferation of fibroblasts was reduced by the spike structures. These effects can result from the cell specific adhesion patterns as shown in this work. These findings show a possibility to design defined surface microstructures, which could control cellular behavior in a cell specific manner.
影响因子：
14
作者：
Yim, EKF;Reano, RM;Leong, KW
通讯作者：
Leong, KW