Iron‐hydroxamate uptake systems in Bacillus subtilis: identification of a lipoprotein as part of a binding protein‐dependent transport system
Iron‐hydroxamate uptake systems in Bacillus subtilis: identification of a lipoprotein as part of a binding protein‐dependent transport system
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枯草芽孢杆菌中的铁异羟肟酸吸收系统：鉴定脂蛋白作为结合蛋白依赖性转运系统的一部分
DOI：
10.1111/j.1365-2958.1993.tb01208.x
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发表时间：
1993
影响因子：
3.6
通讯作者：
K. Hantke
中科院分区：
文献类型：
--
作者：
Richard Schneider;K. Hantke
枯草芽孢杆菌被证明利用三种类型的异羟肟酸铁载体：铁色素、铁胺和shizokinen，每种都被不同的运输系统吸收。分离了铁色素和/或铁胺B吸收缺陷的突变体。克隆了基因 fhuD，该基因能够补充铁色素吸收缺陷的突变体。 FhuD 的推导序列与大肠杆菌的结合蛋白 FepB、FecB 和 FhuD 显示出较低但显着的同源性，这些蛋白都是结合蛋白依赖性铁铁载体转运系统的组成部分。枯草芽孢杆菌FhuD的前23个氨基酸具有脂蛋白信号序列的所有特征。球霉素抑制大肠杆菌中 FhuD 的加工。球霉素的抑制表明大肠杆菌 N 末端半胱氨酸发生脂质修饰。该步骤很可能也发生在枯草芽孢杆菌中。与革兰氏阳性生物体的其他结合蛋白依赖性转运系统一样，有人提出，周质的缺乏可以通过脂质来补偿，结合蛋白通过脂质锚定到细胞质膜上。
Bacillus subtilis was shown to utilize three types of hydroxamate siderophores, ferrichromes, ferrioxamines and shizokinen, each of which is taken up by different transport systems. Mutants deficient in the uptake of ferrichrome and/or ferrioxamine B were isolated. The gene fhuD, which was able to complement a mutant defective in ferrichrome uptake, was cloned. The deduced sequence of FhuD showed low but significant homology to the binding proteins FepB, FecB and FhuD of Escherichia coli, which are all components of binding protein‐dependent, ferric siderophore transport systems. The first 23 amino acids of FhuD of B. subtilis possessed all characteristics of a lipoprotein signal sequence. The processing of FhuD in E. coli was inhibited by globomycin. Inhibition by globomycin indicated a lipid modification at the N‐terminal cysteine in E. coli. It is highly likely that this step may also take place in B. subtilis. As in other binding protein‐dependent transport systems of Gram‐positive organisms it is proposed that the lack of a periplasm is compensated for by the lipid through which the binding protein is anchored to the cytoplasmic membrane.
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DOI：
10.1073/pnas.82.4.1074
发表时间：
1985-01-01
影响因子：
11.1
作者：
TABOR, S;RICHARDSON, CC
通讯作者：
RICHARDSON, CC
鳗弧菌 775 中 pJM1 质粒介导的铁转运系统的分子特征。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Köster,WL;Actis,LA;Waldbeser,LS;Tolmasky,ME;Crosa,JH
通讯作者：
Crosa,JH
Tn917-lac（一种介导枯草芽孢杆菌转录基因融合的转座子衍生物）的构建和特性。
DOI：
10.1073/pnas.83.1.140
发表时间：
1986
影响因子：
11.1
作者：
Perkins,JB;Youngman,PJ
通讯作者：
Youngman,PJ
影响因子：
--
作者：
H. C. Wu;M. Tokunaga
通讯作者：
H. C. Wu;M. Tokunaga
DOI：
10.1073/pnas.87.15.5968
发表时间：
1990-08-01
影响因子：
11.1
作者：
BOYD, J;OZA, MN;MURPHY, JR
通讯作者：
MURPHY, JR