Programmed Cell Death During Secondary Xylem Differentiation in Eucommia ulmoides

Programmed Cell Death During Secondary Xylem Differentiation in Eucommia ulmoides
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杜仲次生木质部分化过程中的程序性细胞死亡

DOI:
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发表时间:
2003-12
期刊:
Acta Bot Sin
影响因子:
--
通讯作者:
Sodmergen
Sodmergen
中科院分区:
其他
文献类型:
--
作者:
Cui Ke-Ming;Cao Jing;He Xin-Qiang;Wang Ya-Qing;Sodmergen

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杜仲次生木质部分化过程中的程序性细胞死亡。通过电子显微镜和DNA断裂和半胱天冬酶样蛋白酶(CLPs)降解的研究对其进行了检测。凝胶电泳法在发育中的次生木质部中检测到DNA阶梯。利用tdt介导的dUTP镍端标记(TUNEL)方法进一步证实DNA断裂。Western blotting分析显示,CLPs (caspase-8和caspase-3样蛋白酶)和PARP(聚adp核糖)聚合酶)在次生木质部分化过程中被降解。结果表明,杜仲次生木质部分化是典型的PCD过程,而CLPs的降解可能是次生木质部分化过程中的组成性PCD事件。
Programmed cell death (PCD) during secondary xylem differentiation in Eucommia ulmoidesOliv. was examined using electron microscopy and by investigation of DNA fragmentation and degradationof caspase-like proteases (CLPs). DNA ladders were detected in developing secondary xylem by gelelectrophoresis. DNA fragmentation was further confirmed by using the TdT-mediated dUTP nick-endlabeling (TUNEL) method. Western blotting analysis showed that CLPs (caspase-8- and caspase-3-likeproteases) and PARP (poly (ADP-ribose) polymerase) were degraded during secondary xylem differentiation.The results thus indicated that secondary xylem differentiation in E. ulmoides was a typical process of PCDand the degradation of CLPs might be a constitutive PCD event during secondary xylem differentiation.
DOI: --
发表时间: 2003
期刊: --
影响因子: --
作者:
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DOI: 10.1111/j.1438-8677.1996.tb00573.x
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