Extraction of Lactobacillus acidophilus CICC 6074 S-Layer Proteins and Their Ability to Inhibit Enteropathogenic Escherichia coli
Extraction of Lactobacillus acidophilus CICC 6074 S-Layer Proteins and Their Ability to Inhibit Enteropathogenic Escherichia coli
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嗜酸乳杆菌 CICC 6074 S 层蛋白的提取及其抑制肠致病性大肠杆菌的能力
DOI：
10.1007/s00284-017-1291-1
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发表时间：
2017-07
影响因子：
2.6
通讯作者：
Pan Daodong
中科院分区：
文献类型：
--
作者：
Zhang Jinye;Gao Jinjin;Guo Yuxing;Wu Zhen;Pan Daodong
从嗜酸乳杆菌菌株CICC 6074中分离到一种与Caco-2细胞结合并抑制肠致病性大肠杆菌（EPEC）的粘附相关蛋白。用5 M LiCl提取S层蛋白，并通过凝胶过滤（G-75）纯化活性级分。通过SDS-PAGE观察S层蛋白，并通过使用质谱估计相对分子量来表征。L.利用Caco-2细胞模型研究了嗜酸乳杆菌及其S层蛋白对EPEC粘附能力的影响。结果表明，L.嗜酸乳杆菌在性质上是粘附性的，并且参与从Caco-2细胞中竞争性排除EPEC。
An adhesion-related protein of Lactobacillus acidophilus strain CICC 6074 involved in binding to Caco-2 cells and inhibiting Enteropathogenic Escherichia coli (EPEC) was isolated and characterized. The S-layer protein was extracted with 5M LiCl and the active fraction purified by gel filtration (G-75). The S-layer protein was visualized by SDS-PAGE and characterized by estimating the relative molecular weight using mass spectra. The inhibitory effect of L. acidophilus and its S-layer proteins on the ability of EPEC to adhere to cells was explored by using a Caco-2 cell model. The results suggest that the S-layer proteins of L. acidophilus are adhesive in nature and are involved in the competitive exclusion of EPEC from Caco-2 cells.
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影响因子：
3.2
作者：
E. Egelseer;I. Schocher;U. Sleytr;M. Sára
通讯作者：
E. Egelseer;I. Schocher;U. Sleytr;M. Sára
影响因子：
3.6
作者：
J. Dworkin;M. Blaser
通讯作者：
J. Dworkin;M. Blaser
DOI：
10.1007/978-3-642-73537-0
发表时间：
1988-10
期刊：
--
影响因子：
--
作者：
Paul Messner;U. Sleytr
通讯作者：
Paul Messner;U. Sleytr
影响因子：
3.2
作者：
Hynönen, U;Westerlund-Wikström, B;Korhonen, TK
通讯作者：
Korhonen, TK
影响因子：
3.4
作者：
KUPCU, S;SARA, M;SLEYTR, UB
通讯作者：
SLEYTR, UB