Subunit Vaccine ESAT-6:c-di-AMP Delivered by Intranasal Route Elicits Immune Responses and Protects Against Mycobacterium tuberculosis Infection.
Subunit Vaccine ESAT-6:c-di-AMP Delivered by Intranasal Route Elicits Immune Responses and Protects Against Mycobacterium tuberculosis Infection.
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亚基疫苗ESAT-6：通过鼻内路线传递的C-DI-AMP会引起免疫反应并预防结核分枝杆菌感染。
DOI：
10.3389/fcimb.2021.647220
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发表时间：
2021
影响因子：
5.7
通讯作者：
Shen L
中科院分区：
文献类型：
--
作者：
Ning H;Zhang W;Kang J;Ding T;Liang X;Lu Y;Guo C;Sun W;Wang H;Bai Y;Shen L
Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb) infection, remains the most common cause of death from a single infectious disease. More safe and effective vaccines are necessary for preventing the prevalence of TB. In this study, a subunit vaccine of ESAT-6 formulated with c-di-AMP (ESAT-6:c-di-AMP) promoted mucosal and systemic immune responses in spleen and lung. ESAT-6:c-di-AMP inhibited the differentiations of CD8+ T cells as well as macrophages, but promoted the differentiations of ILCs in lung. The co-stimulation also enhanced inflammatory cytokines production in MH-S cells. It was first revealed that ESAT-6 and c-di-AMP regulated autophagy of macrophages in different stages, which together resulted in the inhibition of Mtb growth in macrophages during early infection. After Mtb infection, the level of ESAT-6-specific immune responses induced by ESAT-6:c-di-AMP dropped sharply. Finally, inoculation of ESAT-6:c-di-AMP led to significant reduction of bacterial burdens in lungs and spleens of immunized mice. Our results demonstrated that subunit vaccine ESAT-6:c-di-AMP could elicit innate and adaptive immune responses which provided protection against Mtb challenge, and c-di-AMP as a mucosal adjuvant could enhance immunogenicity of antigen, especially for innate immunity, which might be used for new mucosal vaccine against TB.
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影响因子：
3.8
作者：
Matos MN;Cazorla SI;Schulze K;Ebensen T;Guzmán CA;Malchiodi EL
通讯作者：
Malchiodi EL
影响因子：
3.1
作者：
Cooper, AM;DSouza, C;Orme, IM
通讯作者：
Orme, IM
结核分枝杆菌表面蛋白招募泛素来触发宿主异体吞噬
影响因子：
16.6
作者：
Chai, Qiyao;Wang, Xudong;Liu, Cui Hua
通讯作者：
Liu, Cui Hua
影响因子：
3.8
作者：
Jang, Ah-Ra;Choi, Joo-Hee;Park, Jong-Hwan
通讯作者：
Park, Jong-Hwan
重组卡介苗与细菌信号分子环二 AMP 作为内源佐剂在结核分枝杆菌感染后诱导免疫反应升高
影响因子：
7.3
作者：
Ning, Huanhuan;Wang, Lifei;Bai, Yinlan
通讯作者：
Bai, Yinlan