p53 gene mutations in Barrett's epithelium and esophageal cancer.
p53 gene mutations in Barrett's epithelium and esophageal cancer.
复制标题
Barrett 上皮细胞和食管癌中的 p53 基因突变。
作者：
A. Casson;T. Mukhopadhyay;K. Cleary;J. Ro;B. Levin;J. Roth
基因组DNA提取自存档病理学标本，包括10个鳞状细胞癌和14个腺癌，包括7个Barrett上皮邻近肿瘤，和相应的正常食管从切除边缘。使用聚合酶链反应来扩增p53的选定外显子，并使用单链构象多态性分析来分析其突变。1例腺癌和1例鳞状细胞癌和7例Barrett标本中的4例突变定位于外显子8。测序证实了密码子273（CGT-CAT;腺癌）和176（TGC-TTC;鳞状）以及巴雷特上皮密码子152（CCG-CTG）、155（ACC-GCC）和175（CGC-CAC）的突变。来自不同地点的巴雷特上皮标本具有相同的p53突变，表明克隆起源。在突变上皮中产生的癌症没有与巴雷特标本中发现的突变相对应的突变，这表明肿瘤发生需要其他事件。
Genomic DNA was extracted from archival pathology specimens comprising 10 squamous and 14 adenocarcinomas, including 7 with Barrett's epithelium adjacent to tumor, and corresponding normal esophagus from the resection margin. The polymerase chain reaction was used to amplify selected exons of p53 which were analyzed for mutations using single-strand conformation polymorphism analysis. Mutations were localized to exon 8 for 1 adenocarcinoma and to exon 5 for 1 squamous tumor and 4 of 7 Barrett's specimens. Sequencing confirmed mutations at codons 273 (CGT----CAT; adenocarcinoma) and 176 (TGC----TTC; squamous) and in Barrett's epithelium at codons 152 (CCG----CTG), 155 (ACC----GCC) and 175 (CGC----CAC). Specimens of Barrett's epithelium from separate sites had identical p53 mutations suggesting a clonal origin. Cancers arising in mutant epithelium did not have mutations corresponding to those found in the Barrett's specimens suggesting that other events are required for tumorigenesis.
影响因子：
56.9
作者：
MALKIN, D;LI, FP;FRIEND, SH
通讯作者：
FRIEND, SH
甲基苄基亚硝胺诱导的大鼠食管乳头状瘤细胞 Harvey ras 癌基因的突变激活。
影响因子：
11.2
作者：
Wang,Y;You,M;Reynolds,SH;Stoner,GD;Anderson,MW
通讯作者：
Anderson,MW
DOI：
10.1016/0006-291x(85)90390-0
发表时间：
1985-01-01
影响因子：
3.1
作者：
GOELZ, SE;HAMILTON, SR;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B