Concentrations of Fecal Bile Acids in Participants with Functional Gut Disorders and Healthy Controls.
Concentrations of Fecal Bile Acids in Participants with Functional Gut Disorders and Healthy Controls.
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DOI：
10.3390/metabo11090612
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发表时间：
2021-09-09
期刊：
影响因子：
4.1
通讯作者：
Roy NC
中科院分区：
文献类型：
--
作者：
James SC;Fraser K;Young W;Heenan PE;Gearry RB;Keenan JI;Talley NJ;Joyce SA;McNabb WC;Roy NC
胆汁酸是参与营养吸收和信号传递的代谢物，其水平受饮食摄入量、代谢过程和肠道微生物群的影响。我们的目标是量化粪便样本中的23种胆汁酸，以确定健康参与者和功能性肠道疾病患者之间的浓度是否存在差异。用液相色谱-质谱仪(LC-MS)测定了250名罗马IV期IBS(IBS-便秘(C)、IBS-腹泻(D)、IBS混合型(M))、功能性肠道疾病(功能性便秘(FC)、功能性腹泻(FD))和健康对照组(FC n=35，FD n=13，IBS-C n=24，IBS-D n=52，IBS-M n=29，对照组n=97)受试者的粪便胆汁酸。在收集粪便样本之前，记录饮食信息以确定三天的饮食摄入量。所有功能性肠病患者与健康对照组(CDCAp=0.011，CAp=0.003)以及便秘(FC+IBS-C)和腹泻(FD+IBS-D)组之间的粪便胆汁酸(主要是初级胆汁酸)浓度有显著差异(CDCAp=0.001，CAp=0.0002)。胆汁酸在所有功能组间的比较显示，4种代谢物有显著差异，而联合组(FC+IBS-C与FD+IBS-D)的分析显示，10种代谢物有显著差异。FD患者胆汁酸谱与IBS-D相似，FC与IBS-C相似。腹泻表型(FD+IBS-D)患者的胆汁酸浓度高于便秘患者(FC+IBS-C)。胆汁酸代谢产物在功能性肠道疾病患者和健康对照组之间有区别，但在便秘(或腹泻)方面相似，无论是否被归类为IBS。
Bile acids are metabolites involved in nutrient absorption and signaling with levels influenced by dietary intake, metabolic processes, and the gut microbiome. We aimed to quantify 23 bile acids in fecal samples to ascertain if concentrations differed between healthy participants and those with functional gut disorders. Fecal bile acids were measured using liquid chromatography-mass spectrometry (LC-MS) in the COMFORT (The Christchurch IBS cohort to investigate mechanisms for gut relief and improved transit) cohort of 250 participants with Rome IV IBS (IBS-constipation (C), IBS-diarrhea (D), IBS-mixed (M)), functional gut disorders (functional constipation (FC), functional diarrhea (FD)) and healthy controls (FC n = 35, FD n = 13, IBS-C n = 24, IBS-D n = 52, IBS-M n = 29, and control n = 97). Dietary information was recorded to ascertain three-day dietary intake before fecal samples were collected. Fecal bile acid concentrations, predominantly primary bile acids, were significantly different between all functional gut disorder participants and healthy controls (CDCA p = 0.011, CA p = 0.003) and between constipation (FC + IBS-C) and diarrhea (FD + IBS-D) groups (CDCA p = 0.001, CA p = 0.0002). Comparison of bile acids between all functional groups showed four metabolites were significantly different, although analysis of combined groups (FC + IBS-C vs. FD + IBS-D) showed that 10 metabolites were significantly different. The bile acid profiles of FD individuals were similar to those with IBS-D, and likewise, those with FC were similar to IBS-C. Individuals with a diarrhea phenotype (FD + IBS-D) had higher concentrations of bile acids compared to those with constipation (FC + IBS-C). Bile acid metabolites distinguish between individuals with functional gut disorders and healthy controls but are similar in constipation (or diarrhea) whether classified as IBS or not.
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影响因子：
29.4
作者：
Rao AS;Wong BS;Camilleri M;Odunsi-Shiyanbade ST;McKinzie S;Ryks M;Burton D;Carlson P;Lamsam J;Singh R;Zinsmeister AR
通讯作者：
Zinsmeister AR
影响因子：
12.6
作者：
Wong, Banny S.;Camilleri, Michael;Carlson, Paula;McKinzie, Sanna;Busciglio, Irene;Bondar, Olga;Dyer, Roy B.;Lamsam, Jesse;Zinsmeister, Alan R.
通讯作者：
Zinsmeister, Alan R.
DOI：
10.1016/j.cgh.2017.06.039
发表时间：
2018-04
期刊：
Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association
影响因子：
--
作者：
Vijayvargiya P;Busciglio I;Burton D;Donato L;Lueke A;Camilleri M
通讯作者：
Camilleri M
影响因子：
4.2
作者：
Walters, Julian R F;Pattni, Sanjeev S
通讯作者：
Pattni, Sanjeev S
影响因子：
--
作者：
Heenan, Phoebe;Creemers, Rob H;Gearry, Richard B
通讯作者：
Gearry, Richard B