Monoclonal antibody to a proximal nephrogenic renal antigen: immunohistochemical analysis of formalin-fixed, paraffin-embedded human renal cell carcinomas.
Monoclonal antibody to a proximal nephrogenic renal antigen: immunohistochemical analysis of formalin-fixed, paraffin-embedded human renal cell carcinomas.
复制标题
近端肾源性肾抗原的单克隆抗体：福尔马林固定、石蜡包埋的人肾细胞癌的免疫组织化学分析。
作者：
S. O. Yoshida;A. Imam
用人肾皮质组织匀浆的微胞体部分免疫小鼠后，制备了一种针对保存在福尔马林固定、石蜡包埋的肾癌组织切片中的表位的IgG1鼠单抗(PN-15)。对肾细胞癌的固定、包埋组织切片进行免疫组织化学筛查。该抗体在免疫-Western印迹分析中鉴定了一个MR 200,000决定簇，识别的表位位于MR 200,000糖蛋白(Gp200)的碳水化合物结构域中。对36例不同类型的正常肾组织、45例原发性肾细胞癌和38例转移性肾细胞癌，以及39例不同类型的肾肿瘤的124例组织切片进行间接免疫组织化学染色，分析其组织分布。在正常肾组织中，gp200定位于近端小管的刷状边缘和与传出的近端小管相邻的Bowman囊的管腔表面。在乳腺小叶和导管、甲状旁腺和附睾处也发现了相同的组织定位。Gp200在93%的原发和84%的转移性肾癌中表达，主要在细胞表膜上表达。共有抗原表达的其他肿瘤相对较少，包括：乳腺癌、畸胎癌和甲状旁腺腺瘤。PN-15单抗有望成为肾细胞癌固定、包埋病例的回顾调查的有用试剂。该抗体在免疫组织化学诊断中也可能有实用价值，因为它有助于追溯确定转移性透明细胞肿瘤的原发部位，特别是当无法获得此类肿瘤的新鲜冰冻组织时。
An IgG1 murine monoclonal antibody (PN-15) to an epitope preserved in formalin-fixed, paraffin-embedded tissue sections of renal cell carcinomas was developed following immunization of mice with the microsomal fraction of human renal cortical tissue homogenates. An immunohistochemical screening procedure was utilized against fixed, embedded tissue sections of renal cell carcinomas. This antibody identified a Mr 200,000 determinant on immuno-Western blot analysis, and the recognized epitope was shown to reside in the carbohydrate domain of a Mr 200,000 glycoprotein (gp200). Analysis of its tissue distribution was performed by indirect immunoperoxidase staining of fixed, embedded tissue sections of the following: 36 different types of normal tissues; 45 primary and 38 metastatic renal cell carcinomas; and 124 cases of 39 different types of neoplasms. In the normal kidney, gp200 was localized along the brush border of the proximal tubule and the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Shared tissue localization was also noted in breast lobules and ducts, parathyroid gland, and epididymis. The gp200 was expressed by 93% of primary and 84% of metastatic renal cell carcinomas, primarily on the cell surface membrane. Shared antigenic expression was noted in relatively few other tumors including: mammary carcinomas; teratocarcinomas; and parathyroid adenomas. The PN-15 monoclonal antibody holds promise as a useful reagent in retrospective investigations of fixed, embedded cases of renal cell carcinomas. The antibody may also be of practical utility in diagnostic immunohistochemistry by helping to retrospectively define the primary site of metastatic clear cell tumors, especially when fresh frozen tissue from such tumors is not available.
由小鼠单克隆抗体定义的人肾癌细胞表面抗原：组织特异性肾糖蛋白的鉴定。
DOI：
10.1073/pnas.78.8.5122
发表时间：
1981
影响因子：
11.1
作者：
Ueda,R;Ogata,S;Morrissey,DM;Finstad,CL;Szkudlarek,J;Whitmore,WF;Oettgen,HF;Lloyd,KO;Old,LJ
通讯作者：
Old,LJ
针对人肾癌细胞产生的小鼠单克隆抗体所识别的抗原是腺苷脱氨酶结合蛋白。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Andy,RJ;Finstad,CL;Old,LJ;Lloyd,KO;Kornfeld,R
通讯作者：
Kornfeld,R
Lewis 抗原在固定组织中的单克隆抗体定位。
DOI：
--
发表时间：
1984
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Ernst,C;Atkinson,B;Wysocka,M;Blaszczyk,M;Herlyn,M;Sears,H;Steplewski,Z;Koprowski,H
通讯作者：
Koprowski,H