A flow cytometric assay to detect platelet-activating antibodies in VITT after ChAdOx1 nCov-19 vaccination.
A flow cytometric assay to detect platelet-activating antibodies in VITT after ChAdOx1 nCov-19 vaccination.
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DOI：
10.1182/blood.2021012064
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发表时间：
2021-07-01
期刊：
影响因子：
20.3
通讯作者：
Greinacher A
中科院分区：
文献类型：
--
作者：
Handtke S;Wolff M;Zaninetti C;Wesche J;Schönborn L;Aurich K;Ulm L;Hübner NO;Becker K;Thiele T;Greinacher A
The detection of vaccine-induced thrombosis and thrombocytopenia (VITT) is an immediate problem in many parts of the world. VITT shows striking similarities to autoimmune heparin-induced thrombocytopenia, and these conditions need to be distinguished. Handtke et al describe a functional whole-blood flow cytometry test for detection of platelet-activating anti-PF4 antibodies that may be specific for VITT. Anti-PF4 antibodies as detected by some PF4/heparin enzyme immunoassays are not specific for VITT. We provide a functional whole-blood flow cytometry test for detection of platelet-activating anti-PF4 antibodies in VITT. Vaccination is crucial in combatting the severe acute respiratory syndrome coronavirus 2 pandemic. The rare complication of thrombocytopenia and thrombotic complications at unusual sites after ChAdOx1 nCov-19 vaccination is caused by platelet-activating antibodies directed against platelet factor 4 (PF4). We present a widely applicable whole-blood standard flow cytometric assay to identify the pathogenic antibodies associated with vaccine-induced immune-mediated thrombotic thrombocytopenia (VITT) after ChAdOx1 nCov-19 vaccination. This assay will enable rapid diagnosis by many laboratories. This trial was registered at www.clinicaltrials.gov as #NCT04370119.
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DOI：
10.1056/nejmoa2104840
发表时间：
2021-06-03
期刊：
The New England journal of medicine
影响因子：
--
作者：
Greinacher A;Thiele T;Warkentin TE;Weisser K;Kyrle PA;Eichinger S
通讯作者：
Eichinger S
影响因子：
10.4
作者：
Eekels, Julia J. M.;Althaus, Karina;Greinacher, Andreas
通讯作者：
Greinacher, Andreas
影响因子：
158.5
作者：
Greinacher, Andreas
通讯作者：
Greinacher, Andreas
DOI：
10.1056/nejmoa2105385
发表时间：
2021-06-10
期刊：
The New England journal of medicine
影响因子：
--
作者：
Scully M;Singh D;Lown R;Poles A;Solomon T;Levi M;Goldblatt D;Kotoucek P;Thomas W;Lester W
通讯作者：
Lester W
影响因子：
3.1
作者：
Juhl, D;Eichler, P;Greinacher, A
通讯作者：
Greinacher, A