Comparison of [18F]FDG uptake and distribution with hypoxia and proliferation in FaDu human squamous cell carcinoma (hSCC) xenografts after single dose irradiation
Comparison of [18F]FDG uptake and distribution with hypoxia and proliferation in FaDu human squamous cell carcinoma (hSCC) xenografts after single dose irradiation
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单剂量照射后FaDu人鳞状细胞癌（hSCC）异种移植物中[18F]FDG摄取和分布与缺氧和增殖的比较
DOI：
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发表时间：
2009
影响因子：
2.6
通讯作者：
B. Beuthien
中科院分区：
文献类型：
--
作者：
Kerstin Bruechner;R. Bergmann;A. Santiago;B. Mosch;A. Yaromina;F. Hessel;F. Hofheinz;J. van den Hoff;M. Baumann;B. Beuthien
目的：本研究采用正电子发射断层扫描（PET）、放射自显影和功能组织学研究了单次剂量照射后早期时间点人肿瘤异种移植FaDu中[18 F]2-氟-2-脱氧葡萄糖（[18 F]FDG）的摄取。材料与方法：在25戈伊或35戈伊单剂量照射之前和照射后1、7或11天（p. p.）进行裸鼠上FaDu hSCC异种移植物的[18 F]FDG-PET。在第二次PET之前，给小鼠注射匹莫硝唑（pimonidazole，pimo）和溴脱氧尿苷（BrdU）。PET肿瘤切除后，切片并进行放射自显影和功能性组织学染色（pimo，BrdU，Ki 67）。[18 F]FDG肿瘤摄取在PET扫描中通过最大标准摄取值（SUVmax）进行定量，并在与组织学切片共配准后进行放射自显影。结果：PET或放射自显影显示，两个剂量组和时间点之间的[18 F]FDG总摄取量无差异。比较放射自显影和组织学，[18 F]FDG摄取是恒定的肿瘤坏死随着时间的推移，而它在重要的肿瘤区域，特别是在缺氧区域下降。Ki 67和BrdU的阳性和阴性区域之间的[18 F]FDG摄取无差异被发现。结论：[18 F]FDG摄取的下降在重要的肿瘤和pimo阳性区域中看到的放射自显影，不反映由PET确定的SUVmax的评价。这些发现表明，SUVmax并不一定反映照射后肿瘤生物学的变化。
Purpose: This study investigated the uptake of [18F]2-fluoro-2-deoxy-glucose ([18F]FDG) in the human tumour xenograft FaDu at early time points after single dose irradiation with Positron-Emission-Tomography (PET), autoradiography and functional histology. Materials and methods: [18F]FDG-PET of FaDu hSCC xenografts on nude mice was performed before 25 Gy or 35 Gy single dose irradiation and one, seven or 11 days post irradiation (p.irr.). Before the second PET, mice were injected with pimonidazole (pimo) and bromodeoxyuridine (BrdU). After the PET tumours were excised, sliced and subjected to autoradiography and functional histology staining (pimo, BrdU, Ki67). [18F]FDG tumour uptake was quantified in the PET scans by maximal standard uptake value (SUVmax) and in the autoradiography after co-registration to the histology slices. Results: No differences in the overall [18F]FDG uptake between the two dose groups and time points were found with PET or autoradiography. Comparing autoradiography and histology, the [18F]FDG uptake was constant in tumour necrosis over time, while it decreased in vital tumour areas and particularly in hypoxic regions. No differences in the [18F]FDG uptake between positive and negative areas of Ki67 and BrdU were found. Conclusions: The decline of [18F]FDG uptake in vital tumour and in pimopositive areas as seen in autoradiography, was not reflected by evaluation of SUVmax determined by PET. These findings suggest that the SUVmax does not necessarily reflect changes in tumour biology after irradiation.
DOI：
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发表时间：
1995-09
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
--
作者：
A. Clavo;Raya S. Brown;R. Wahl
通讯作者：
A. Clavo;Raya S. Brown;R. Wahl
放射自显影评估人卵巢腺癌异种移植物中 2-脱氧-D-葡萄糖和单克隆抗体的瘤内分布。
DOI：
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发表时间：
1993
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
--
作者：
Brown,RS;Fisher,SJ;Wahl,RL
通讯作者：
Wahl,RL
DOI：
10.1016/j.ijrobp.2005.02.009
发表时间：
2005-06-01
影响因子：
7
作者：
Pugachev, A;Ruan, S;Humm, JL
通讯作者：
Humm, JL