Assembly of an active microbial consortium by engineering compatible combinations containing foreign and native biocontrol bacteria of kiwifruit.
Assembly of an active microbial consortium by engineering compatible combinations containing foreign and native biocontrol bacteria of kiwifruit.
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通过含有猕猴桃的外来和本地生防细菌的工程相容组合组装活性微生物聚生体。
DOI：
10.1016/j.csbj.2023.07.021
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发表时间：
2023
影响因子：
6
通讯作者：
中科院分区：
文献类型：
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作者：
组装功能性细菌生防聚生体有望扩大生防剂的范围和效率。通常，细菌种间相互作用导致不相容性事件，因为细菌可以产生抗菌化合物和/或组装接触依赖性杀伤（CDK）装置。在这里，我们的目的是组装一个细菌财团包括溶杆菌产酶OH 11和芽孢杆菌safensis ZK-1的协同控制的细菌和真菌病害的猕猴桃。ZK-1是一种天然的猕猴桃生防细菌，对猕猴桃假单胞菌致病变种（Pseudomonaseparingae pv.）猕猴桃溃疡病病原菌放线菌素（Psa），但抗真菌活性较弱。OH 11是一种具有较强抑菌活性的国外猕猴桃生防菌剂。虽然OH 11不能产生抗革兰氏阴性菌的代谢产物，但该菌株可以利用IV型分泌系统作为抗菌CDK武器。我们首先观察到OH 11可以通过产生可扩散的抗革兰氏阳性抗生素WAP-8294 A2来抑制ZK-1的生长，而ZK-1不能产生可扩散的抗菌化合物来抑制OH 11的生长。为了破坏这种种间不相容性，我们通过删除WAP-8294 A2生物合成基因产生了转基因OH 11衍生菌株OH 11 W，并发现OH 11 W不能杀死ZK-1。我们进一步观察到，当OH 11 W和ZK-1在琼脂平板上共接种时，它们之间没有观察到CDK效应，而OH 11 W或ZK-1与Psa在琼脂平板上共培养则导致Psa死亡，表明L.酶基因和B. safensis组装了针对细菌病原体的抗菌CDK武器，并且这些CDK武器不影响OH 11 W与ZK-1的相容性。基于这些发现，我们组装了一个依赖于OH 11 W/ZK-1的聚生体，该聚生体被证明在控制猕猴桃细菌性溃疡病和几种代表性真菌病害中具有功能。
Assembling functional bacterial biocontrol consortia is expected to expand the scope and efficiency of biocontrol agents. Generally, bacterial interspecies interactions lead to incompatibility events, as bacteria can produce antibacterial compounds and/or assemble contact-dependent killing (CDK) devices. Here, we aimed to assemble a bacterial consortium comprising Lysobacter enzymogenes OH11 and Bacillus safensis ZK-1 for the synergistic control of bacterial and fungal diseases of kiwifruit. ZK-1, a native kiwifruit biocontrol bacterium, is effective against Pseudomonas syringae pv. actinidiae (Psa) that causes bacterial kiwifruit canker, but has weak antifungal activity. OH11 is a foreign kiwifruit biocontrol agent with strong antifungal activity. While OH11 was unable to produce anti-Gram-negative metabolites, this strain could utilize type IV secretion system as an antibacterial CDK weapon. We first observed that OH11 could inhibit growth of ZK-1 by generating diffusible anti-Gram-positive antibiotic WAP-8294A2, whereas ZK-1 failed to generate diffusible antibacterial compound to inhibit growth of OH11. To disrupt this interspecies incompatibility, we generated a transgenic OH11-derived strain, OH11W, by deleting the WAP-8294A2 biosynthetic gene and found that OH11W did not kill ZK-1. We further observed that when OH11W and ZK-1 were co-inoculated on agar plates, no CDK effect was observed between them, whereas co-culture of OH11W or ZK-1 with Psa on agar plates resulted in Psa killing, suggesting L. enzymogenes and B. safensis assemble antibacterial CDK weapons against bacterial pathogens, and these CDK weapons did not affect the compatibility between OH11W and ZK-1. Based on these findings, we assembled an OH11W/ZK-1 dependent consortium that was shown to be functional in controlling bacterial canker and several representative fungal diseases of kiwifruit.
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影响因子：
4.1
作者：
Fira, Djordje;Dimkic, Ivica;Stankovic, Slavisa
通讯作者：
Stankovic, Slavisa
DOI：
10.1038/nrmicro2259
发表时间：
2010-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1016/j.ijfoodmicro.2018.04.024
发表时间：
2018-08-02
影响因子：
5.4
作者：
Li, Wusun;Yuan, Shuzhi;Cao, Jiankang
通讯作者：
Cao, Jiankang
DOI：
10.1038/ismej.2016.169
发表时间：
2017-04
期刊：
The ISME journal
影响因子：
--
作者：
Bernal P;Allsopp LP;Filloux A;Llamas MA
通讯作者：
Llamas MA
影响因子：
--
作者：
Qian Guo-liang;Hu Bai-shi;Liu Feng-quan
通讯作者：
Liu Feng-quan