CircPOMT1 and circMCM3AP inhibit osteogenic differentiation of human adipose-derived stem cells by targeting miR-6881-3p.
CircPOMT1 and circMCM3AP inhibit osteogenic differentiation of human adipose-derived stem cells by targeting miR-6881-3p.
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CircPOMT1 和 circMCM3AP 通过靶向 miR-6881-3p 抑制人脂肪干细胞的成骨分化。
DOI：
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发表时间：
2019-08
影响因子：
2.2
通讯作者：
Zhi-He Zhao
中科院分区：
文献类型：
--
作者：
Xin-Qi Huang;Xiao Cen;Wen-Tian Sun;Kai Xia;Li-Yuan Yu;Jun Liu;Zhi-He Zhao
环状rna （Circular RNAs, circRNAs）是一种新型的内源性非编码rna，具有特殊的环状结构，在各种组织和疾病的发育中发挥着重要作用。然而，很少有研究关注环状rna在人脂肪源性干细胞（hASCs）成骨中的功能和机制。在这里，我们进行了环状rna测序和生物信息学分析，以研究成骨分化过程中hASCs的表达谱。有150个上调的环状rna和60个下调的环状rna差异表达。其中，circPOMT1和circMCM3AP在hASCs成骨过程中表达下调。hsa-miR-6881-3p能促进hASCs的成骨分化，而circPOMT1和circMCM3AP的表达与之呈负相关。Smad6和Chordin是bmp信号通路的关键抑制剂，预计是hsa-miR-6881-3p的靶标。因此，circPOMT1和circMCM3AP可能通过bmp信号通路靶向hsa-miR-6881-3p，从而影响hASCs的成骨分化。CircPOMT1和circMCM3AP是骨缺损修复的潜在新靶点。
Circular RNAs (circRNAs), novel endogenous non-coding RNAs with the special circular structure, have been found to play critical roles in various development of tissues and diseases. However, few studies have focused on the functions and mechanisms of circRNAs in the osteogenesis of human adipose-derived stem cells (hASCs). Here, we performed the circRNAs sequencing and bioinformatic analysis to investigate the expression profiles of hASCs during osteogenic differentiation. There were 150 upregulated circRNAs and 60 downregulated circRNAs expressed differentially. Among them, the expression of circPOMT1 and circMCM3AP were downregulated during the osteogenesis of hASCs. hsa-miR-6881-3p could promote the osteogenic differentiation of hASCs, while the expression of circPOMT1 and circMCM3AP were negatively correlated with it. Smad6 and Chordin, critical inhibitors of the BMPs signaling pathway, were predicted to be the targets of hsa-miR-6881-3p. Therefore, circPOMT1 and circMCM3AP might influence the osteogenic differentiation of hASCs by targeting hsa-miR-6881-3p via BMPs signaling pathway. CircPOMT1 and circMCM3AP are potential novel targets for the repairment of bone defects.
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发表时间：
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