Development of PCR for identifying Streptococcus parasuis, a close relative of Streptococcus suis.
Development of PCR for identifying Streptococcus parasuis, a close relative of Streptococcus suis.
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PCR的开发用于鉴定parpococcus paraSuis，suis链球菌的亲戚。
DOI：
10.1292/jvms.18-0083
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发表时间：
2018-07-12
期刊：
影响因子：
--
通讯作者：
Sekizaki T
中科院分区：
文献类型：
--
作者：
Yamada R;Tien LHT;Arai S;Tohya M;Ishida-Kuroki K;Nomoto R;Kim H;Suzuki E;Osawa R;Watanabe T;Sekizaki T
最近，副猪链球菌已从分类学上从人畜共患病病原体猪链球菌中删除。在健康猪和患病猪中均检测到副猪链球菌，这表明副猪链球菌参与猪的正常微生物群并具有潜在的致病性。然而，由于缺乏区分副猪链球菌和猪链球菌的适当检测方法，副猪链球菌对猪的致病性尚不清楚。在这项研究中，我们开发了一种针对副猪链球菌的特异性 PCR 方法。 PCR 的检测限为每个反应 350 CFU。收集从八头猪的唾液中分离出的细菌并通过 PCR 进行检查。从所有猪的样本中获得了 64 个 PCR 阳性分离株。 64株菌株中，有13株经基因证实为副猪链球菌，其生物学和生化特征与已知副猪链球菌菌株几乎相同，表明PCR阳性菌株为副猪链球菌。在 64 个分离株中，28 个分离株是猪链球菌血清型分型方案中的血清型 20、22 或 26。其余 36 个分离株无法分型，这表明存在新的血清型或胶囊阴性形式。因此，本研究中描述的PCR方法是鉴定副猪链球菌的有用工具，并且可用于该细菌的病原学研究。
Streptococcus parasuis has recently been removed taxonomically from Streptococcus suis, a zoonotic pathogen. S. parasuis has been detected in healthy pigs and in diseased pigs, which suggests that S. parasuis is involved in the normal microbiota of pigs and has potential pathogenicity. However, the pathogenicity of S. parasuis in pigs is unclear because of the lack of appropriate detection methods that discriminate S. parasuis from S. suis. In this study, we developed a PCR method that is specific for S. parasuis. The detection limit of the PCR was 350 CFU per reaction. Bacteria isolated from the saliva of eight pigs were collected and examined by PCR. Sixty-four isolates positive for PCR were obtained from the samples of all pigs. Thirteen of the 64 isolates were genetically confirmed as S. parasuis, and biologically and biochemically had nearly the same features of known S. parasuis strains, which suggested that strains positive for PCR were S. parasuis. Among the 64 isolates, 28 isolates were serotypes 20, 22, or 26 in the S. suis serotyping scheme. The remaining 36 isolates were untypeable, which suggested the presence of novel serotypes or a capsule-negative form. Therefore, the PCR method described in this study is a useful tool for identifying S. parasuis, and can be used in etiological studies on this bacterium.
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加拿大病猪分离的非血清型猪链球菌的基因分型和荚膜多糖合成基因位点研究
影响因子：
4.4
作者：
Zheng H;Qiu X;Roy D;Segura M;Du P;Xu J;Gottschalk M
通讯作者：
Gottschalk M
影响因子：
3.3
作者：
Le Hong Thuy Tien;Nishibori, Tomoyuki;Osawa, Ro
通讯作者：
Osawa, Ro
影响因子：
9.4
作者：
GOTTSCHALK, M;HIGGINS, R;HENRICHSEN, J
通讯作者：
HENRICHSEN, J
DOI：
10.1099/ijs.0.067116-0
发表时间：
2015-02-01
影响因子：
2.8
作者：
Nomoto, R.;Maruyama, F.;Osawa, Ro
通讯作者：
Osawa, Ro
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro