Recent Molecular Tools for the Genetic Manipulation of Highly Industrially Important Mucoromycota Fungi.
Recent Molecular Tools for the Genetic Manipulation of Highly Industrially Important Mucoromycota Fungi.
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DOI：
10.3390/jof7121061
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发表时间：
2021-12-10
期刊：
影响因子：
--
通讯作者：
Song Y
中科院分区：
文献类型：
--
作者：
Mohamed H;Naz T;Yang J;Shah AM;Nazir Y;Song Y
毛霉目（Mucorales）是毛霉门（Mucormycota）中最大和研究最充分的目，以其快速生长速度和各种工业应用而闻名。毛霉目真菌是一组迷人的丝状生物，在研究、工业和医学领域具有许多用途。它们是广泛使用的各种次级代谢产物和其他增值产品的生物技术生产商。毛霉目的某些成员被广泛用作遗传和分子研究的模式生物，并扩展了我们对该目其他成员代谢的理解。与其他真菌物种相比，我们对毛霉属真菌的了解仍处于起步阶段，这可能与缺乏有效的遗传工具有关。然而，分子工具和方法的最新进展，例如可回收标记的构建，沉默载体和基于CRISPR-Cas9的基因编辑系统，帮助我们修改了这些模式生物的基因组。多种遗传修饰已被证明可以大规模产生有价值的产品，并帮助我们了解毛霉真菌的形态发生，基本生物学，发病机制和宿主-病原体相互作用。在这篇综述中，我们讨论了各种传统的和现代的遗传工具和方法，用于有效的基因修饰在工业上重要的成员毛霉目。
Mucorales is the largest and most well-studied order of the phylum Mucormycota and is known for its rapid growth rate and various industrial applications. The Mucorales fungi are a fascinating group of filamentous organisms with many uses in research and the industrial and medical fields. They are widely used biotechnological producers of various secondary metabolites and other value-added products. Certain members of Mucorales are extensively used as model organisms for genetic and molecular investigation and have extended our understanding of the metabolisms of other members of this order as well. Compared with other fungal species, our understanding of Mucoralean fungi is still in its infancy, which could be linked to their lack of effective genetic tools. However, recent advancements in molecular tools and approaches, such as the construction of recyclable markers, silencing vectors, and the CRISPR-Cas9-based gene-editing system, have helped us to modify the genomes of these model organisms. Multiple genetic modifications have been shown to generate valuable products on a large scale and helped us to understand the morphogenesis, basic biology, pathogenesis, and host–pathogen interactions of Mucoralean fungi. In this review, we discuss various conventional and modern genetic tools and approaches used for efficient gene modification in industrially important members of Mucorales.
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DOI：
10.1007/bf02174347
发表时间：
1996-04-24
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
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Alic N;Hoddinott MP;Foley A;Slack C;Piper MD;Partridge L
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Partridge L
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10.2174/2213235x04666160407212156
发表时间：
2016-08
期刊：
Current Metabolomics
影响因子：
--
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影响因子：
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Cervantes M;Vila A;Nicolás FE;Moxon S;de Haro JP;Dalmay T;Torres-Martínez S;Ruiz-Vázquez RM
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