A single argonaute gene participates in exogenous and endogenous RNAi and controls cellular functions in the basal fungus Mucor circinelloides.
A single argonaute gene participates in exogenous and endogenous RNAi and controls cellular functions in the basal fungus Mucor circinelloides.
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DOI：
10.1371/journal.pone.0069283
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ruiz-Vázquez RM
中科院分区：
文献类型：
--
作者：
Cervantes M;Vila A;Nicolás FE;Moxon S;de Haro JP;Dalmay T;Torres-Martínez S;Ruiz-Vázquez RM
RNAi的机制在后生动物中有很好的描述，它在多种细胞功能中发挥作用。然而，尽管已经在真菌中鉴定了不同类别的内源性小RNA（esRNA），但它们的生物学作用描述得很差，部分原因是缺乏在这些esRNA的生物发生中受影响的突变体的表型。Argonaute蛋白是RNAi途径的关键组分之一，其中该蛋白家族的不同成员参与广泛的esRNA分子库的生物发生。在这里，我们确定了三个argonaute基因的真菌卷枝毛霉，并调查他们参与外源性和内源性RNAi。我们发现，只有一个ago基因，ago-1，在营养生长过程中参与RNAi，并需要转基因诱导的RNA沉默和积累不同类别的esRNA来源于外显子（ex-siRNA）。I类和II类ex-siRNA与Ago-1结合以控制靶蛋白编码基因的mRNA积累。III类ex-siRNA不特异性结合Ago-1，但需要这种蛋白质来产生它们，揭示了ex-siRNA生物合成途径的复杂性。我们还表明，ago-1参与了对环境信号的反应，因为营养胁迫诱导的营养发育和自溶受到ago-1 − M的影响。卷枝藻突变体我们的研究结果表明，一个单一的Ago蛋白参与了通过不同途径产生的不同类别的esRNA的产生。他们还强调了ex-siRNA在真菌内源基因调控中的作用，并扩大了RNAi调节的生物学功能范围。
The mechanism of RNAi is well described in metazoans where it plays a role in diverse cellular functions. However, although different classes of endogenous small RNAs (esRNAs) have been identified in fungi, their biological roles are poorly described due, in part, to the lack of phenotype of mutants affected in the biogenesis of these esRNAs. Argonaute proteins are one of the key components of the RNAi pathways, in which different members of this protein family participate in the biogenesis of a wide repertoire of esRNAs molecules. Here we identified three argonaute genes of the fungus Mucor circinelloides and investigated their participation in exogenous and endogenous RNAi. We found that only one of the ago genes, ago-1, is involved in RNAi during vegetative growth and is required for both transgene-induced RNA silencing and the accumulation of distinct classes of esRNAs derived from exons (ex-siRNAs). Classes I and II ex-siRNAs bind to Ago-1 to control mRNA accumulation of the target protein coding genes. Class III ex-siRNAs do not specifically bind to Ago-1, but requires this protein for their production, revealing the complexity of the biogenesis pathways of ex-siRNAs. We also show that ago-1 is involved in the response to environmental signals, since vegetative development and autolysis induced by nutritional stress are affected in ago-1 − M. circinelloides mutants. Our results demonstrate that a single Ago protein participates in the production of different classes of esRNAs that are generated through different pathways. They also highlight the role of ex-siRNAs in the regulation of endogenous genes in fungi and expand the range of biological functions modulated by RNAi.
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影响因子：
11.4
作者：
Nicolás, FE;Torres-Martínez, S;Ruiz-Vázquez, RM
通讯作者：
Ruiz-Vázquez, RM
影响因子：
10.5
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影响因子：
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通讯作者：
Gascuel O
影响因子：
64.8
作者：
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通讯作者：
Sattler, M
影响因子：
64.5
作者：
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通讯作者：
Qi, Yijun