Covalent cross-linking of growth hormone-releasing factor to pituitary receptors.
Covalent cross-linking of growth hormone-releasing factor to pituitary receptors.
复制标题
生长激素释放因子与垂体受体的共价交联。
作者：
G. Velicelebi;S. Patthi;S. Provow;M. Akong
We have used a bifunctional cross-linker, disuccinimidyl suberate, to covalently attach [125I]human pancreatic GH-releasing factor (GHRF) (-1-40)OH to bovine pituitary membranes and rat anterior pituitary cells. Covalently radiolabeled membrane and cell preparations were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing and nonreducing conditions. In the former case, we observed the specific labeling of a polypeptide with an apparent mol wt of 75,000 +/- 3,000. The labeling of this species was specific for GHRF, as evidenced by the fact that it was inhibited in a dose-dependent fashion with increasing concentration of unlabeled GHRF. Furthermore, the radiolabeling was inhibited in the presence of excess unlabeled GHRF analogs but not unrelated peptides such as insulin and rat GH. The size of the radiolabeled band was the same in both bovine pituitary membranes and rat anterior pituitary cells. The extent of radiolabeling was dependent on the amount of membrane or the number of cells present during the binding reaction. These observations indicate that the mol wt 75,000 species is a ligand-binding subunit of the GHRF receptor in the pituitary. Under nonreducing conditions, a species much larger than mol wt 200,000 was specifically radiolabeled, again in both bovine pituitary membranes and rat cells. This result suggests the possibility that the ligand-binding subunit might be disulfide-linked to other subunit(s) forming homo- and heterooligomers.
登录
查看更多内容
血管活性肠多肽与其完整人淋巴母细胞上的受体的共价交联。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wood,CL;O'Dorisio,MS
通讯作者：
O'Dorisio,MS
来自人胰腺肿瘤的具有生长激素释放活性的 40 残基肽的表征。
DOI：
10.1016/0006-291x(82)91578-9
发表时间：
1982
影响因子：
3.1
作者：
Esch,FS;Böhlen,P;Ling,NC;Brazeau,PE;Wehrenberg,WB;Thorner,MO;Cronin,MJ;Guillemin,R
通讯作者：
Guillemin,R
用于阐明生长激素释放因子类似物的结构-活性关系的极其灵敏的体外模型。
影响因子：
4.8
作者：
Heiman,ML;Nekola,MV;Murphy,WA;Lance,VA;Coy,DH
通讯作者：
Coy,DH
影响因子：
56.9
作者：
GUILLEMIN, R;BRAZEAU, P;WEHRENBERG, WB
通讯作者：
WEHRENBERG, WB
合成人胰腺（肿瘤）GH 释放因子和生长抑素、三碘甲腺原氨酸和地塞米松对体外 GH 分泌的影响。
影响因子：
4.8
作者：
Vale,W;Vaughan,J;Yamamoto,G;Spiess,J;Rivier,J
通讯作者：
Rivier,J