Acinetobacter baumannii utilizes a type VI secretion system for bacterial competition.
Acinetobacter baumannii utilizes a type VI secretion system for bacterial competition.
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DOI：
10.1371/journal.pone.0059388
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Munson RS Jr
中科院分区：
文献类型：
--
作者：
Carruthers MD;Nicholson PA;Tracy EN;Munson RS Jr
VI型分泌系统(T6SS)是一类大分子分泌机器，被许多细菌用于细菌间的竞争或在真核细胞中引发反应。鲍曼不动杆菌是一种在人类中引起严重感染的机会性病原体。这些感染，包括肺炎和菌血症，很重要，因为它们往往与医院和医疗机构有关，在这些地方，它们不成比例地影响危重病人，如住在重症监护病房的病人。虽然已知鲍曼不动杆菌基因组携带的基因预测产物与其他细菌的T6SS相关基因产物具有同源性，并且已证明可分泌主要的T6SS结构蛋白Hcp，但有关鲍曼不动杆菌T6SS的进一步研究尚未见报道。在此，我们证明了鲍曼不动杆菌M2株分泌Hcp，并且这种分泌依赖于TSSB，TSSB是一个噬菌体收缩鞘蛋白的同源蛋白，证实了M2株产生了一个有功能的T6SS。此外，我们还证明了菌株M2的竞争能力依赖于TSSB和HCP的产物。总之，我们的数据为鲍曼不动杆菌产生的T6SS在细菌间竞争中的功能提供了第一个证据。
Type VI secretion systems (T6SS) are a class of macromolecular secretion machines that are utilized by a number of bacteria for inter-bacterial competition or to elicit responses in eukaryotic cells. Acinetobacter baumannii is an opportunistic pathogen that causes severe infections in humans. These infections, including pneumonia and bacteremia, are important, as they are often associated with hospitals and medical-settings where they disproportionally affect critically ill patients like those residing in intensive care units. While it is known that A. baumannii genomes carry genes whose predicted products have homology with T6SS-associated gene products from other bacteria, and secretion of a major T6SS structural protein Hcp has been demonstrated, no additional work on an A. baumannii T6SS has been reported. Herein, we demonstrated that A. baumannii strain M2 secretes Hcp and this secretion was dependent upon TssB, an ortholog of a bacteriophage contractile sheath protein, confirming that strain M2 produces a functional T6SS. Additionally, we demonstrated that the ability of strain M2 to out-compete Escherichia coli was reliant upon the products of tssB and hcp. Collectively, our data have provided the first evidence demonstrating function in inter-bacterial competition, for a T6SS produced by A. baumannii.
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影响因子：
9.9
作者：
de Berardinis, Veronique;Vallenet, David;Castelli, Vanina;Besnard, Marielle;Pinet, Agnes;Cruaud, Corinne;Samair, Sumitta;Lechaplais, Christophe;Gyapay, Gabor;Richez, Celine;Durot, Maxime;Kreimeyer, Annett;Le Fevre, Francois;Schaechter, Vincent;Pezo, Valerie;Doering, Volker;Scarpelli, Claude;Medigue, Claudine;Cohen, Georges N.;Marliere, Philippe;Salanoubat, Marcel;Weissenbach, Jean
通讯作者：
Weissenbach, Jean
影响因子：
3.1
作者：
Gaddy, Jennifer A.;Arivett, Brock A.;Actis, Luis A.
通讯作者：
Actis, Luis A.
影响因子：
48
作者：
Choi, KH;Gaynor, JB;Schweizer, HP
通讯作者：
Schweizer, HP
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
14.8
作者：
Kelley, Lawrence A.;Sternberg, Michael J. E.
通讯作者：
Sternberg, Michael J. E.