A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1.-猫眼课题宝

DOI：

10.1038/msb.2008.10

发表时间：

2008

期刊：

MOLECULAR SYSTEMS BIOLOGY

影响因子：

9.9

通讯作者：

中科院分区：

生物学1区

文献类型：

作者：

de Berardinis, Veronique;Vallenet, David;Castelli, Vanina;Besnard, Marielle;Pinet, Agnes;Cruaud, Corinne;Samair, Sumitta;Lechaplais, Christophe;Gyapay, Gabor;Richez, Celine;Durot, Maxime;Kreimeyer, Annett;Le Fevre, Francois;Schaechter, Vincent;Pezo, Valerie;Doering, Volker;Scarpelli, Claude;Medigue, Claudine;Cohen, Georges N.;Marliere, Philippe;Salanoubat, Marcel;Weissenbach, Jean

关键词：

Acinetobacter baylyi ADP1 deletion mutants essential genes growth phenotype metabolism systems biology

来源链接：

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290942/?report=classic

我们构建了一个土壤细菌鲍氏不动杆菌ADP1所有非必需基因的单基因缺失突变体集合。共获得了2594个缺失突变体，而有499个（16%）未获得，因此这些是在基本培养基上生存的候选必需基因。这个必需性数据集与从在基本培养基上生长情况进行分析的庆应突变体集合所推断出的大肠杆菌数据集有88%的一致性，而在铜绿假单胞菌中被描述为必需的直系同源基因中有80%在ADP1中也是必需的。我们采取了几种策略来研究ADP1的代谢：（1）寻找我们的必需性数据与当前代谢知识之间的差异；（2）将这个必需性数据集与来自其他生物的数据集进行比较；（3）对突变体集合在多种碳源（奎尼酸、2 - 3丁二醇、葡萄糖等）上进行系统表型分析。这个集合为通过正向和反向遗传学方法研究基因功能提供了一种新的资源，并为系统生物学方法构成了一个可靠的实验数据源。

We have constructed a collection of single-gene deletion mutants for all dispensable genes of the soil bacterium Acinetobacter baylyi ADP1. A total of 2594 deletion mutants were obtained, whereas 499 (16%) were not, and are therefore candidate essential genes for life on minimal medium. This essentiality data set is 88% consistent with the Escherichia coli data set inferred from the Keio mutant collection profiled for growth on minimal medium, while 80% of the orthologous genes described as essential in Pseudomonas aeruginosa are also essential in ADP1. Several strategies were undertaken to investigate ADP1 metabolism by (1) searching for discrepancies between our essentiality data and current metabolic knowledge, (2) comparing this essentiality data set to those from other organisms, (3) systematic phenotyping of the mutant collection on a variety of carbon sources (quinate, 2-3 butanediol, glucose, etc.). This collection provides a new resource for the study of gene function by forward and reverse genetic approaches and constitutes a robust experimental data source for systems biology approaches.