Eukaryotic protein glycosylation: a primer for histochemists and cell biologists.
Eukaryotic protein glycosylation: a primer for histochemists and cell biologists.
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DOI：
10.1007/s00418-016-1526-4
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发表时间：
2017-02
影响因子：
2.3
通讯作者：
Corfield A
中科院分区：
文献类型：
--
作者：
Corfield A
蛋白质经历共翻译和翻译后修饰，并且它们的糖基化是最常见和结构多样化的类型。在组织化学上，首先通过染色剂检测聚糖的存在。碳水化合物特异性工具（凝集素，单克隆抗体）的可用性已经彻底改变了糖表型，允许监测不同的结构。描述了真核生物中不同类型的蛋白质糖基化。在这个教育调查之后，给出了已知生物功能与蛋白质携带的聚糖结构相关的例子。特别地，粘蛋白及其糖基化模式被认为是指导性的原理证明案例。本文综述了糖蛋白生物合成和代谢的组织和细胞定位，并着重介绍了杯状细胞中糖蛋白的新发现。最后，通过选定的例子记录了疾病中的蛋白质糖基化，其中异常聚糖表达影响正常功能，使疾病病理变得明显。在这些研究中采用的组织学应用强调整个文本。
Proteins undergo co- and posttranslational modifications, and their glycosylation is the most frequent and structurally variegated type. Histochemically, the detection of glycan presence has first been performed by stains. The availability of carbohydrate-specific tools (lectins, monoclonal antibodies) has revolutionized glycophenotyping, allowing monitoring of distinct structures. The different types of protein glycosylation in Eukaryotes are described. Following this educational survey, examples where known biological function is related to the glycan structures carried by proteins are given. In particular, mucins and their glycosylation patterns are considered as instructive proof-of-principle case. The tissue and cellular location of glycoprotein biosynthesis and metabolism is reviewed, with attention to new findings in goblet cells. Finally, protein glycosylation in disease is documented, with selected examples, where aberrant glycan expression impacts on normal function to let disease pathology become manifest. The histological applications adopted in these studies are emphasized throughout the text.
影响因子：
4.4
作者：
Hunt PN;Wilson MD;von Schalburg KR;Davidson WS;Koop BF
通讯作者：
Koop BF