Expression and genomic organization of zonadhesin-like genes in three species of fish give insight into the evolutionary history of a mosaic protein.
Expression and genomic organization of zonadhesin-like genes in three species of fish give insight into the evolutionary history of a mosaic protein.
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DOI：
10.1186/1471-2164-6-165
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发表时间：
2005-11-22
期刊：
影响因子：
4.4
通讯作者：
Koop BF
中科院分区：
文献类型：
--
作者：
Hunt PN;Wilson MD;von Schalburg KR;Davidson WS;Koop BF
镶嵌精子蛋白zonadhesin（ZAN）的特点是在哺乳动物和物种特异性卵精子结合的相互作用。zonadhesin的基因组结构和睾丸特异性表达是已知的许多哺乳动物物种。迄今为止，所有zonadhesin基因的特征在于由meprin A5抗原受体酪氨酸磷酸酶mu（MAM）结构域，粘蛋白串联重复序列，和冯维勒布兰德（VWD）粘附结构域。在这里，我们调查的基因组结构和zonadhesin样基因在三种鱼类的表达。对大西洋鲑（萨尔莫salar）zonadhesin-like基因（zlg）的cDNA和相应的基因组位点进行了测序。Zlg在粘附结构域内容上与哺乳动物zonadhesin相似;然而，结构域顺序改变。分析河豚（红鳍东方鲀）和斑马鱼（斑马鱼）的序列数据确定zonadhesin（zan）基因，共享相同的结构域顺序，内容，和一个保守的同线关系与哺乳动物zonadhesin。在D.雷里奥也被指认。与哺乳动物zonadhesin不同，D. rerio zan和S. Salar ZLG在肠中表达而在睾丸中不表达。我们在T. rubripes和D. rerio和在S. salar和D.雷里奥。这些基因中的每一个都含有MAM、粘蛋白和VWD结构域。虽然这些结构域与几种显示出显著肠道表达的蛋白质相关，但它们的组合对于脊椎动物中的zonadhesin和zonadhesin样基因是独特的。鱼类zonadhesin和zonadhesin-like基因的表达模式表明，zonadhesin的生殖作用在哺乳动物谱系中进化较晚。
The mosaic sperm protein zonadhesin (ZAN) has been characterized in mammals and is implicated in species-specific egg-sperm binding interactions. The genomic structure and testes-specific expression of zonadhesin is known for many mammalian species. All zonadhesin genes characterized to date consist of meprin A5 antigen receptor tyrosine phosphatase mu (MAM) domains, mucin tandem repeats, and von Willebrand (VWD) adhesion domains. Here we investigate the genomic structure and expression of zonadhesin-like genes in three species of fish. The cDNA and corresponding genomic locus of a zonadhesin-like gene (zlg) in Atlantic salmon (Salmo salar) were sequenced. Zlg is similar in adhesion domain content to mammalian zonadhesin; however, the domain order is altered. Analysis of puffer fish (Takifugu rubripes) and zebrafish (Danio rerio) sequence data identified zonadhesin (zan) genes that share the same domain order, content, and a conserved syntenic relationship with mammalian zonadhesin. A zonadhesin-like gene in D. rerio was also identified. Unlike mammalian zonadhesin, D. rerio zan and S. salar zlg were expressed in the gut and not in the testes. We characterized likely orthologs of zonadhesin in both T. rubripes and D. rerio and uncovered zonadhesin-like genes in S. salar and D. rerio. Each of these genes contains MAM, mucin, and VWD domains. While these domains are associated with several proteins that show prominent gut expression, their combination is unique to zonadhesin and zonadhesin-like genes in vertebrates. The expression patterns of fish zonadhesin and zonadhesin-like genes suggest that the reproductive role of zonadhesin evolved later in the mammalian lineage.
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通讯作者：
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