Novel cleavage of reductively aminated glycan-tags by N-bromosuccinimide to regenerate free, reducing glycans.
Novel cleavage of reductively aminated glycan-tags by N-bromosuccinimide to regenerate free, reducing glycans.
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DOI：
10.1021/cb400513k
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发表时间：
2013-11-15
影响因子：
4
通讯作者：
Cummings, Richard D.
中科院分区：
文献类型：
--
作者：
Song, Xuezheng;Johns, Brian A.;Ju, Hong;Lasanajak, Yi;Zhao, Chunmei;Smith, David F.;Cummings, Richard D.
Glycans that are fluorescently tagged by reductive amination have been useful for functional glycomic studies. However, the existing tags can introduce unwanted properties to the glycans and complicate structural and functional studies. Here we describe a facile method using N-bromosuccinimide (NBS) to remove the tags and efficiently regenerate free reducing glycans. The regenerated free reducing glycans can be easily analyzed by routine mass spectrometry or re-tagged with different tags for further studies. This new method can be used to efficiently remove a variety of fluorescent tags installed by reductive amination, including 2-aminobenzoic acid and 2-aminopyridine. NBS treatment essentially transforms the commonly used 2-aminobenzoic linkage to a cleavable linkage. It can be used to cleave printed glycans from microarrays and cleave neoglycopeptides containing a 2-aminobenzoic linker.
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影响因子：
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通讯作者：
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影响因子：
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通讯作者：
MATSUSHIMA, Y
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