Biosynthetic Nanobubble-Mediated CRISPR/Cas9 Gene Editing of Cdh2 Inhibits Breast Cancer Metastasis.
Biosynthetic Nanobubble-Mediated CRISPR/Cas9 Gene Editing of Cdh2 Inhibits Breast Cancer Metastasis.
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生物合成纳米气泡介导的 CRISPR/Cas9 基因编辑 Cdh2 抑制乳腺癌转移
DOI：
10.3390/pharmaceutics14071382
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发表时间：
2022-06-30
期刊：
影响因子：
5.4
通讯作者：
Liu, Jianhua
中科院分区：
文献类型：
--
作者：
Gao, Ruru;Luo, Qiong;Li, Yang;Song, Liming;Cai, Junnan (Stephen);Xiong, Ying;Yan, Fei;Liu, Jianhua
上皮-间充质转化（EMT）是上皮细胞经历一系列生化变化以获得间充质表型的过程，其与肿瘤转移有关。在这里，我们提出了一种敲除EMT相关Cdh 2基因的新策略，该基因通过CRISPR/Cas9介导的基因编辑编码N-钙粘蛋白，该基因通过超声与生物合成纳米泡（Gas Vesicles，GV）相结合。使用聚乙烯亚胺作为基因递送载体以将sgRNA递送到稳定表达Cas9蛋白的4 T1细胞中，产生稳定的Cdh 2基因敲除细胞系。Western印迹分析证实了在这些Cdh 2基因敲除的4 T1细胞系中不存在N-钙粘蛋白蛋白。与野生型细胞相比，在Cdh 2基因敲除的4 T1细胞中观察到肿瘤细胞迁移显着减少。我们的研究表明，超声结合GV可以有效介导Cdh 2基因的CRISPR/Cas9基因编辑，以抑制肿瘤的侵袭和转移。
The epithelial-mesenchymal transition (EMT), a process in which epithelial cells undergo a series of biochemical changes to acquire a mesenchymal phenotype, has been linked to tumor metastasis. Here, we present a novel strategy for knocking out the EMT-related Cdh2 gene, which encodes N-cadherin through CRISPR/Cas9-mediated gene editing by an ultrasound combined with biosynthetic nanobubbles (Gas Vesicles, GVs). Polyethyleneimine were employed as a gene delivery vector to deliver sgRNA into 4T1 cells that stably express the Cas9 protein, resulting in the stable Cdh2 gene- knockout cell lines. The Western blotting assay confirmed the absence of an N-cadherin protein in these Cdh2 gene-knockout 4T1 cell lines. Significantly reduced tumor cell migration was observed in the Cdh2 gene-knockout 4T1 cells in comparison with the wild-type cells. Our study demonstrated that an ultrasound combined with GVs could effectively mediate CRISPR/Cas9 gene editing of a Cdh2 gene to inhibit tumor invasion and metastasis.
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影响因子：
16.6
作者：
Mancuso P;Chen C;Kaminski R;Gordon J;Liao S;Robinson JA;Smith MD;Liu H;Sariyer IK;Sariyer R;Peterson TA;Donadoni M;Williams JB;Siddiqui S;Bunnell BA;Ling B;MacLean AG;Burdo TH;Khalili K
通讯作者：
Khalili K
影响因子：
158.5
作者：
Xu, Lei;Wang, Jun;Chen, Hu
通讯作者：
Chen, Hu
DOI：
10.3390/life5010385
发表时间：
2015-02-02
期刊：
Life (Basel, Switzerland)
影响因子：
--
作者：
Pfeifer F
通讯作者：
Pfeifer F
影响因子：
1.6
作者：
Cai, Junhong;Huang, Sizhe;Bao, Shan
通讯作者：
Bao, Shan
DOI：
10.1073/pnas.1512503112
发表时间：
2015-08-18
影响因子：
11.1
作者：
Schumann, Kathrin;Lin, Steven;Marson, Alexander
通讯作者：
Marson, Alexander