Gluconate 5-dehydrogenase (Ga5DH) participates in Streptococcus suis cell division.
Gluconate 5-dehydrogenase (Ga5DH) participates in Streptococcus suis cell division.
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DOI：
10.1007/s13238-014-0074-8
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发表时间：
2014-10
期刊：
影响因子：
21.1
通讯作者：
Gao, George F.
中科院分区：
文献类型：
--
作者：
Shi, Zhongyu;Xuan, Chunling;Han, Huiming;Cheng, Xia;Wang, Jundong;Feng, Youjun;Srinivas, Swaminath;Lu, Guangwen;Gao, George F.
细菌细胞分裂在相等子细胞的形成中受到严格调节。这个过程受到一系列空间和时间调节器的控制，并且最近已经确定了该领域感兴趣的几个新因素。在此，我们报告了人畜共患病原体猪链球菌的细胞分裂对葡萄糖酸 5-脱氢酶 (Ga5DH) 的需求。 Ga5DH 催化 5-酮葡萄糖酸可逆还原为 D-葡萄糖酸，并定位于细胞分裂位点。猪链球菌中 Ga5DH 的缺失导致后代形态丰满，隔膜异常。还观察到细胞长度显着增加。这些缺陷被确定为猪链球菌中 Ga5DH 缺失导致 FtsZ 离域的结果。此外，通过蛋白质相互作用测定证实了 FtsZ 与 Ga5DH 的体外相互作用。这些结果表明 Ga5DH 可能起到防止猪链球菌细胞分裂过程中异位 Z 环形成的作用。
Bacterial cell division is strictly regulated in the formation of equal daughter cells. This process is governed by a series of spatial and temporal regulators, and several new factors of interest to the field have recently been identified. Here, we report the requirement of gluconate 5-dehydrogenase (Ga5DH) in cell division of the zoonotic pathogen Streptococcus suis. Ga5DH catalyzes the reversible reduction of 5-ketogluconate to D-gluconate and was localized to the site of cell division. The deletion of Ga5DH in S. suis resulted in a plump morphology with aberrant septa joining the progeny. A significant increase was also observed in cell length. These defects were determined to be the consequence of Ga5DH deprivation in S. suis causing FtsZ delocalization. In addition, the interaction of FtsZ with Ga5DH in vitro was confirmed by protein interaction assays. These results indicate that Ga5DH may function to prevent the formation of ectopic Z rings during S. suis cell division.
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影响因子：
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影响因子：
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通讯作者：
Gao GF