Microfluidic cytometer based on dual photodiode detection for cell size and deformability analysis.
Microfluidic cytometer based on dual photodiode detection for cell size and deformability analysis.
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基于双光电二极管检测的微流控细胞仪，用于细胞尺寸和变形性分析。
DOI：
10.1016/j.talanta.2013.03.004
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发表时间：
2013
期刊：
影响因子：
6.1
通讯作者：
J. D. den Toonder
中科院分区：
文献类型：
--
作者：
Qin Ji;G. Du;Martijn J van Uden;Q. Fang;J. D. den Toonder
细胞力学特性在疾病诊断中起着重要作用。根据细胞的力学性质区分细胞为无标记诊断提供了一种潜在的方法。本工作以低成本商用激光器为光源，以两个光电二极管为探测器，研制了一种方便、低成本的微流控细胞仪，用于研究细胞力学性质和细胞大小随传输强度的变化。细胞通过一个宽度小于细胞尺寸的狭窄微通道，集成在聚二甲基硅氧烷芯片中，激光聚焦在下面。单个细胞的传输时间是通过两个光电二极管检测的时间差来测量的。利用该装置研究了细胞松弛素D处理前后HL60细胞力学性质的差异，并通过测量透射率来区分不同直径的细胞，即HL60细胞和红细胞。
Cellular mechanical properties play an important role in disease diagnosis. Distinguishing cells based on their mechanical properties provides a potential method for label-free diagnosis. In this work, a convenient and low-cost microfluidic cytometer was developed to study cell mechanical properties and cell size based on the change of transmission intensity, using a low-cost commercial laser as a light source and two photodiodes as detectors. The cells pass through a narrow microchannel with a width smaller than the cell dimension, integrated in a polydimethylsiloxane chip, below which the laser is focused. The transit time of individual cells is measured by the time difference detected by two photodiodes. This device was used to study the difference in cell mechanical properties between HL60 cells treated with and without Cytochalasin D. Furthermore, it was also applied to distinguish cells with different diameters, HL60 cells and red blood cells, by measuring the transmission intensity.
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影响因子：
56.9
作者：
Unger, MA;Chou, HP;Quake, SR
通讯作者：
Quake, SR
影响因子：
20.3
作者：
Glenister, FK;Coppel, RL;Cooke, BM
通讯作者：
Cooke, BM
DOI：
10.1021/la803413x
发表时间：
2009-07-07
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Hohne DN;Younger JG;Solomon MJ
通讯作者：
Solomon MJ
影响因子：
9.7
作者：
Suresh, Subra
通讯作者：
Suresh, Subra
DOI：
10.1073/pnas.96.3.921
发表时间：
1999-02-02
影响因子：
11.1
作者：
Rotsch, C;Jacobson, K;Radmacher, M
通讯作者：
Radmacher, M