Fungal community structure in disease suppressive soils assessed by 28S LSU gene sequencing.
Fungal community structure in disease suppressive soils assessed by 28S LSU gene sequencing.
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DOI：
10.1371/journal.pone.0093893
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Roget DK
中科院分区：
文献类型：
--
作者：
Penton CR;Gupta VV;Tiedje JM;Neate SM;Ophel-Keller K;Gillings M;Harvey P;Pham A;Roget DK
土壤传播疾病的自然生物抑制是土壤微生物群落活性和组成的功能。土壤微生物和植物病原体的相互作用可发生在作物播种前和/或根际，随后影响植物生长和生产力。虽然真菌也可以影响土传疾病，但对抑制微生物群落的研究主要集中在细菌上。利用针对真菌28S LSU rRNA基因的454焦磷酸测序技术，在南澳大利亚两个地点的土壤中对真菌进行了“抑制”或“非抑制”的分析。每个重复至少从125 g土壤中提取DNA，以减少微尺度群落变异，并从播种时和7周根际采集的土壤样本中提取DNA，以覆盖根丝核菌感染高峰期。共有约994,000个reads被划分为917个属，覆盖了RDP真菌分类器数据库的54%，对于碱性，低有机质土壤来说具有很高的多样性。统计分析和群落排序显示，抑菌土壤和非抑菌土壤以及不同土壤类型/位置间真菌群落组成存在显著差异。抑制土壤的差异主要集中在不到40个属，包括一些具有植物病原菌抑制潜力的内生物种和支寄生虫，如Xylaria spp，非抑制土壤的差异主要集中在Alternaria、Gibberella和Penicillum。焦磷酸测序生成了真菌群落结构的详细描述，并确定了可能在稳定的疾病抑制中影响病原体-植物相互作用的候选分类群。
Natural biological suppression of soil-borne diseases is a function of the activity and composition of soil microbial communities. Soil microbe and phytopathogen interactions can occur prior to crop sowing and/or in the rhizosphere, subsequently influencing both plant growth and productivity. Research on suppressive microbial communities has concentrated on bacteria although fungi can also influence soil-borne disease. Fungi were analyzed in co-located soils ‘suppressive’ or ‘non-suppressive’ for disease caused by Rhizoctonia solani AG 8 at two sites in South Australia using 454 pyrosequencing targeting the fungal 28S LSU rRNA gene. DNA was extracted from a minimum of 125 g of soil per replicate to reduce the micro-scale community variability, and from soil samples taken at sowing and from the rhizosphere at 7 weeks to cover the peak Rhizoctonia infection period. A total of ∼994,000 reads were classified into 917 genera covering 54% of the RDP Fungal Classifier database, a high diversity for an alkaline, low organic matter soil. Statistical analyses and community ordinations revealed significant differences in fungal community composition between suppressive and non-suppressive soil and between soil type/location. The majority of differences associated with suppressive soils were attributed to less than 40 genera including a number of endophytic species with plant pathogen suppression potentials and mycoparasites such as Xylaria spp. Non-suppressive soils were dominated by Alternaria, Gibberella and Penicillum. Pyrosequencing generated a detailed description of fungal community structure and identified candidate taxa that may influence pathogen-plant interactions in stable disease suppression.
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影响因子：
4.2
作者：
Garnica, Sigisfredo;Riess, Kai;Weiss, Michael
通讯作者：
Weiss, Michael
影响因子：
4.4
作者：
Campbell, CD;Chapman, SJ;Potts, JM
通讯作者：
Potts, JM
DOI：
10.2136/sssaj1992.03615995005600030017x
发表时间：
1992-05-01
影响因子：
2.9
作者：
CAMBARDELLA, CA;ELLIOTT, ET
通讯作者：
ELLIOTT, ET
影响因子：
4.9
作者：
GARDES, M;BRUNS, TD
通讯作者：
BRUNS, TD
影响因子：
4.4
作者：
Gottel, Neil R.;Castro, Hector F.;Schadt, Christopher W.
通讯作者：
Schadt, Christopher W.