Product binding enforces the genomic specificity of a yeast polycomb repressive complex.
Product binding enforces the genomic specificity of a yeast polycomb repressive complex.
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DOI：
10.1016/j.cell.2014.11.039
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发表时间：
2015-01-15
期刊：
影响因子：
64.5
通讯作者：
Madhani HD
中科院分区：
文献类型：
--
作者：
Dumesic PA;Homer CM;Moresco JJ;Pack LR;Shanle EK;Coyle SM;Strahl BD;Fujimori DG;Yates JR 3rd;Madhani HD
我们的Polycomb系统的特点，组装阻遏性的亚端粒结构域的H3K27甲基化（H3K27me）在酵母新生隐球菌。这种PRC2样蛋白复合物的纯化揭示了动物PRC2组分的直向同源物以及含有染色体结构域的亚基Ccc1，其识别H3K27me。然而，EZH或EED直系同源物的去除消除了H3K27 me，Ccc 1的标记识别的破坏导致H3K27 me重新分布。引人注目的是，H3K27me的结果模式与H3K9me标记的异染色质结构域一致。事实上，C.新生儿H3K9甲基转移酶Clr4导致H3K9me和重新分布的H3K27me标记的丢失。这些研究结果表明，锚定的染色质修饰复合物，其产品抑制其吸引力不同的染色质类型，解释酶的作用组蛋白，这往往窝藏产品识别模块，可能存款不同的染色质结构域，尽管共享一个高度丰富的和基本相同的底物核小体。
We characterize the Polycomb system that assembles repressive subtelomeric domains of H3K27 methylation (H3K27me) in the yeast Cryptococcus neoformans. Purification of this PRC2-like protein complex reveals orthologs of animal PRC2 components as well as a chromodomain-containing subunit, Ccc1, which recognizes H3K27me. Whereas removal of either the EZH or EED ortholog eliminates H3K27me, disruption of mark recognition by Ccc1 causes H3K27me to redistribute. Strikingly, the resulting pattern of H3K27me coincides with domains of heterochromatin marked by H3K9me. Indeed, additional removal of the C. neoformans H3K9 methyltransferase Clr4 results in loss of both H3K9me and the redistributed H3K27me marks. These findings indicate that the anchoring of a chromatin-modifying complex to its product suppresses its attraction to a different chromatin type, explaining how enzymes that act on histones, which often harbor product recognition modules, may deposit distinct chromatin domains despite sharing a highly abundant and largely identical substrate—the nucleosome.
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DOI：
10.1073/pnas.1303750110
发表时间：
2013-04-09
影响因子：
11.1
作者：
Jamieson, Kirsty;Rountree, Michael R.;Selker, Eric U.
通讯作者：
Selker, Eric U.
影响因子：
10.7
作者：
Chow, Eve W. L.;Morrow, Carl A.;Fraser, James A.
通讯作者：
Fraser, James A.
影响因子：
8.8
作者：
Klein BJ;Piao L;Xi Y;Rincon-Arano H;Rothbart SB;Peng D;Wen H;Larson C;Zhang X;Zheng X;Cortazar MA;Peña PV;Mangan A;Bentley DL;Strahl BD;Groudine M;Li W;Shi X;Kutateladze TG
通讯作者：
Kutateladze TG
影响因子：
16.8
作者：
Collins, Robert E.;Northrop, Jeffrey P.;Cheng, Xiaodong
通讯作者：
Cheng, Xiaodong
影响因子：
64.5
作者：
Liou, GG;Tanny, JC;Moazed, D
通讯作者：
Moazed, D