Male-killing toxin in a bacterial symbiont of Drosophila.
Male-killing toxin in a bacterial symbiont of Drosophila.
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DOI：
10.1038/s41586-018-0086-2
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发表时间：
2018-05
期刊：
影响因子：
64.8
通讯作者：
Lemaitre B
中科院分区：
文献类型：
--
作者：
Harumoto T;Lemaitre B
昆虫中的几个共生细菌谱系自私地操纵宿主繁殖，以在一个种群中传播，通常是通过扭曲宿主的性别比例。普氏螺旋体是一种螺旋状、可移动的革兰氏阳性共生菌，广泛存在于果蝇中。螺旋体的一个显著特征是杀死男性，即在发育过程中有选择地杀死受感染的女性宿主的儿子。虽然自20世纪50年代以来，人们对螺旋体引起的男性死亡进行了研究，但其潜在的机制尚不清楚。在这里，我们发现了一种螺旋体蛋白，命名为SpAID，它的表达可以诱导男性死亡。SpAID在黑腹螺旋体中的过表达会杀死雄性，但不会杀死雌性，并诱导大量的细胞凋亡和神经缺陷，这概括了在感染螺旋体的男性胚胎中观察到的病理。我们的数据表明，SpAID针对男性X染色体上的剂量补偿机制来调节其影响。SpAID含有锚蛋白重复序列和脱泛素酶结构域，这是其亚细胞定位和活性所必需的。此外，我们还发现了一株实验室突变株螺旋体，其对男性的杀伤力降低，SpAID基因座存在较大缺失。总而言之，我们的研究发现了一种新的细菌蛋白，它以性别特有的方式影响宿主细胞器，这可能是人们长期寻找的导致螺旋体诱导男性死亡的因素。
Several lineages of symbiotic bacteria in insects selfishly manipulate host reproduction to spread in a population, often by distorting host sex ratios. Spiroplasma poulsonii (hereafter Spiroplasma) is a helical and motile, Gram-positive symbiotic bacterium, that resides in a wide range of Drosophila species. A striking feature of Spiroplasma is male killing, whereby the sons of infected female hosts are selectively killed during development. Although male killing caused by Spiroplasma has been studied since the 1950s, the underlying mechanism is unknown. Here, we identify a Spiroplasma protein, designated SpAID, whose expression induces male killing. Overexpression of SpAID in D. melanogaster kills males but not females, and induces massive apoptosis and neural defects, recapitulating the pathology observed in Spiroplasma-infected male embryos. Our data suggest that SpAID targets the dosage compensation machinery on the male X chromosome to mediate its effects. SpAID contains ankyrin repeats and a deubiquitinase domain, which are required for its subcellular localization and activity. Moreover, we found a laboratory mutant strain of Spiroplasma with reduced male-killing ability and a large deletion in the SpAID locus. Collectively, our study has uncovered a novel bacterial protein that affects host cellular machinery in a sex-specific way, which is likely to be the long-searched-for factor responsible for Spiroplasma-induced male killing.
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影响因子：
15.9
作者：
Al-Khodor S;Price CT;Kalia A;Abu Kwaik Y
通讯作者：
Abu Kwaik Y
影响因子：
16.6
作者：
Harumoto T;Anbutsu H;Lemaitre B;Fukatsu T
通讯作者：
Fukatsu T
影响因子：
10.5
作者：
BOPP, D;BELL, LR;SCHEDL, P
通讯作者：
SCHEDL, P
影响因子：
6.7
作者：
Harumoto T;Anbutsu H;Fukatsu T
通讯作者：
Fukatsu T
DOI：
10.1016/j.cub.2016.03.050
发表时间：
2016-05-23
期刊：
Current biology : CB
影响因子：
--
作者：
Cheng B;Kuppanda N;Aldrich JC;Akbari OS;Ferree PM
通讯作者：
Ferree PM