Effects on Fitness Components of Enhanced Expression of Elongation Factor EF-1α in Drosophila melanogaster. I. The Contrasting Approaches of Molecular and Population Biologists
Effects on Fitness Components of Enhanced Expression of Elongation Factor EF-1α in Drosophila melanogaster. I. The Contrasting Approaches of Molecular and Population Biologists
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延长因子 EF-1α 增强表达对果蝇健康成分的影响 I. 分子生物学家和群体生物学家的对比方法。
DOI：
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发表时间：
1993
影响因子：
2.9
通讯作者：
E. Hillesheim
中科院分区：
文献类型：
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作者：
S. Stearns;M. Kaiser;E. Hillesheim
我们测量了在较高温度下延长因子1α（EF-1α）基因（一种已知生理功能的基因）表达增强的交配雌性果蝇的寿命和繁殖力，并将这些测量结果与在25 ℃和29.5 ℃下对对照系进行的类似测量结果进行了比较。我们还测量了对处女男性和女性寿命的类似影响。通过因子ANOVA和考克斯回归分析中处理-温度相互作用效应的显著性，检验了29.5 ℃时EF-1α表达增强可延长寿命的假设。该效应仅对交配雌性动物具有显著性。与对照组雌性相比，EF-1α表达增强的给药组雌性的寿命显著延长，且在生命早期产卵显著减少，这一结果并非由EF-1α对体重的可能影响介导。有一个独立的处理与温度的相互作用的影响。未处理雌性在25 ℃和29.5 ℃时的寿命显著延长。与先前的结果相反，EF-1α的表达增强对处女男性的寿命没有影响。的进化理论的患病和偏见引入实验设计通过特定的理论的影响进行了讨论。
We measured the life spans and fecundities of mated female fruit flies that had enhanced expression at higher temperatures of the gene for elongation factor 1α (EF-1α), a gene of known physiological function, and compared those measurements with similar measurements made on control lines at 25⚬ and 29.5 ⚬C. We also measured similar effects on the life spans of virgin males and females. The hypothesis that enhanced expression of EF-1α at 29.5⚬C increases life span was tested by the significance of the treatment-by-temperature interaction effect in factorial ANOVAs and Cox regressions. This effect was significant for mated females only. Treatment females with enhanced expression of EF-1α lived significantly longer and laid significantly fewer eggs early in life than did control females, a result not mediated by possible effects of EF-1α on body weight. There was an effect of treatment independent of the interaction with temperature. Virgin treatment females lived significantly longer at both 25⚬ and 29.5⚬. In contrast to previous results, enhanced expression of EF-1α had no impact on the life spans of virgin males. Implications are discussed for the evolutionary theory of ailing and for biases introduced into experimental design through the influence of particular theories.
影响因子：
56.9
作者：
JOHNSON, TE
通讯作者：
JOHNSON, TE
DOI：
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发表时间：
1972
期刊：
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影响因子：
--
作者：
D. Cox
通讯作者：
D. Cox