Serum antiglycan antibody detection of nonmucinous ovarian cancers by using a printed glycan array.
Serum antiglycan antibody detection of nonmucinous ovarian cancers by using a printed glycan array.
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DOI：
10.1002/ijc.26002
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发表时间：
2012-01-01
影响因子：
6.4
通讯作者：
Heinzelmann-Schwarz, Viola
中科院分区：
文献类型：
--
作者：
Jacob, Francis;Goldstein, Darlene R.;Bovin, Nicolai V.;Pochechueva, Tatiana;Spengler, Marianne;Caduff, Rosemarie;Fink, Daniel;Vuskovic, Marko I.;Huflejt, Margaret E.;Heinzelmann-Schwarz, Viola
在妇科癌症中，上皮性卵巢癌的死亡率最高。糖基化改变与产生肿瘤相关碳水化合物抗原的致癌转化有关。我们利用印刷聚糖阵列研究了天然抗聚糖抗体在卵巢癌诊断中的潜力。采用生物素-链亲和素荧光系统检测了203种化学合成的印刷聚糖结合的抗聚糖抗体，这些抗体存在于手术表现正常的女性（健康对照，n=24）和FIGO分期不同的非黏液性交界性癌或卵巢癌（n=33）的血清中。测量已知ABO血型的二糖、三糖和四糖抗原，验证数据。抗多糖抗体具有高重复性（rc>0.9）。聚类分析确定了特定核心碳水化合物结构的重复模式：11个n -链聚糖，3个o -链聚糖，2个鞘糖脂。生物标志物检测显示，包括P1 （Galα1-4Galβ1-4GlcNAcβ, p<0.001）在内的24种聚糖在（低）恶性肿瘤和健康对照之间具有显著区别。通过一组多变量选择和线性联合抗聚糖抗体信号，获得了与肿瘤标志物CA125相当的敏感性和特异性（分别为79.2%和84.8%）。我们的发现证明了聚糖阵列在开发新一代卵巢癌生物标志物方面的潜力。
Epithelial Ovarian cancer has the highest mortality rate among gynaecological cancers. Altered glycosylation is associated with oncogenic transformation producing tumor-associated carbohydrate antigens. We investigated the potential of natural occurring anti-glycan antibodies in the diagnosis of ovarian cancer by using printed glycan array. Anti-glycan antibodies bound to 203 chemically synthesized printed glycans were detected via biotin-streptavidin fluorescence system in serum of women with normal operative findings (healthy controls; n=24) and non-mucinous borderline or ovarian cancer of various FIGO stages (n=33). Data were validated measuring blood group associated di-, tri and tetra- saccharide antigens on known ABO blood groups. Anti-glycan antibodies demonstrated high reproducibility (rc>0.9). Cluster analysis identified repetitive patterns of specific core carbohydrate structures: 11 N-linked glycans, 3 O-linked glycans, 2 glycosphingolipids. Biomarker detection revealed 24 glycans including P1 (Galα1-4Galβ1-4GlcNAcβ; p<0.001) significantly discriminating between (low-) malignant tumors and healthy controls. Comparable sensitivity and specificity with tumor marker CA125 was achieved by a panel of multivariate selected and linear combined anti-glycan antibody signals (79.2% and 84.8%, respectively). Our findings demonstrate the potential of glycan arrays in the development of a new generation of biomarkers for ovarian cancer.
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影响因子：
8.8
作者：
通讯作者：
--
影响因子：
3.4
作者：
Heinzelmann-Schwarz, Viola A.;Scolyer, Richard A.;O'Brien, Philippa M.
通讯作者：
O'Brien, Philippa M.
影响因子：
2.6
作者：
Dotan, N.;Altstock, R. T.;Dukler, A.
通讯作者：
Dukler, A.
影响因子：
4.7
作者：
Moore, Richard G.;Brown, Amy K.;Bast, Robert C., Jr.
通讯作者：
Bast, Robert C., Jr.
影响因子：
2.2
作者：
Jacob, Francis;Goldstein, Darlene R.;Heinzelmann-Schwarz, Viola
通讯作者：
Heinzelmann-Schwarz, Viola