Fimbriae-associated genes are biofilm-forming factors in Aggregatibacter actinomycetemcomitans strains.
Fimbriae-associated genes are biofilm-forming factors in Aggregatibacter actinomycetemcomitans strains.
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菌毛相关基因是放线聚集菌菌株中的生物膜形成因子。
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
K. Sakurai
中科院分区：
文献类型：
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作者：
T. Saito;K. Ishihara;M. Ryu;K. Okuda;K. Sakurai
放线菌聚集菌在人类牙周病变中定植，与侵袭性牙周炎和慢性牙周炎都有关系。临床分离的放线菌属菌落为粗糙型。粗糙型有一种非凡的能力，可以顽强地附着在固体表面上，并牢固地殖民。在反复接种过程中，粗糙型菌落转变为光滑型菌落，生物膜形成活性停止。放线菌的粘附是由紧密粘附（tad）基因位点介导的，该基因位点包括flp、rcpA和rcpB。在本研究中，我们研究了其生物膜形成能力与与菌膜蛋白产生相关的flp、rcpA和rcpB基因表达的关系。首先，我们将玻璃表面的粗糙型菌种组织生物膜转变为光滑型菌种组织生物膜，并通过观察玻璃表面的生物膜进行证实。然后，我们进行了Real-Time PCR，发现在光滑型菌落中rcpA和rcpB基因的表达明显降低。这表明rcpA和rcpB的表达在放线菌菌的生物膜形成和牙周病变持续感染的建立中起着关键作用。
Aggregatibactor actinomycetemcomitans colonizes human periodontal lesions and is implicated in both aggressive periodontitis and chronic periodontitis. Clinical isolated colonies of A. actinomycetemcomitans were rough type. The rough type has a remarkable ability to adhere tenaciously to solid surfaces and colonize firmly. Rough type colonies change into smooth type colonies during the course of repeated inoculation and biofilm-forming activity ceases. Adherence by A. actinomycetemcomitans is mediated by the tight-adherence (tad) gene locus, which includes flp, rcpA and rcpB. In this study, we investigated the relationship between its biofilm-forming ability and expression of the flp, rcpA and rcpB genes associated with fimbriae protein production. First, we changed rough type strain organized biofilm on glass into smooth type and confirmed it by observation of biofilm on glass surfaces. Then, we carried out Real-Time PCR and found that expression of the rcpA and rcpB genes was clearly reduced in smooth type colonies. This suggests that expression of rcpA and rcpB plays a key role in biofilm formation by A. actinomycetemcomitans strains and the establishment of persistent infections in periodontal lesions.
齿垢密螺旋体的 43 kDa 蛋白对于牙菌素活性至关重要。
影响因子：
2.1
作者：
Ishihara,Kazuyuki;Kuramitsu,HowardK;Okuda,Katsuji
通讯作者：
Okuda,Katsuji
影响因子：
3
作者：
Haase, EM;Bonstein, T;Scannapieco, FA
通讯作者：
Scannapieco, FA