Untangling the R2* contrast in multiple sclerosis: A combined MRI-histology study at 7.0 Tesla.
Untangling the R2* contrast in multiple sclerosis: A combined MRI-histology study at 7.0 Tesla.
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DOI：
10.1371/journal.pone.0193839
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Welch EB
中科院分区：
文献类型：
--
作者：
Bagnato F;Hametner S;Boyd E;Endmayr V;Shi Y;Ikonomidou V;Chen G;Pawate S;Lassmann H;Smith S;Welch EB
T2*加权多回波梯度回声磁共振成像及其倒数R2*由于其对铁含量的敏感性而被用于脑成像。对于表现出铁和髓磷脂含量病理改变的多发性硬化症患者，使用R2*可能提供一种独特的方法来解开疾病的机制。采用全身7.0特斯拉MRI扫描仪对8例多发性硬化症患者的脑冠状面切片进行成像。扫描方案包括三维（3D） T2*-w多回波梯度回波和二维T2*-w涡轮自旋回波（TSE）序列。髓磷脂和铁含量的组织病理学分析采用Luxol快速蓝和蛋白脂质髓磷脂染色和3,3 ' -四盐酸二氨基联苯胺增强特恩布尔蓝染色。定量R2*、髓磷脂和铁强度。研究发现，在多发性硬化症大脑的不同区域，髓磷脂和铁含量对R2*的影响是不同的。这些数据将为临床研究人员解决T2*/R2*变异作为MS患者大脑组织完整性生物标志物的作用提供参考。
T2*-weighted multi-echo gradient-echo magnetic resonance imaging and its reciprocal R2* are used in brain imaging due to their sensitivity to iron content. In patients with multiple sclerosis who display pathological alterations in iron and myelin contents, the use of R2* may offer a unique way to untangle mechanisms of disease. Coronal slices from 8 brains of deceased multiple sclerosis patients were imaged using a whole-body 7.0 Tesla MRI scanner. The scanning protocol included three-dimensional (3D) T2*-w multi-echo gradient-echo and 2D T2-w turbo spin echo (TSE) sequences. Histopathological analyses of myelin and iron content were done using Luxol fast blue and proteolipid myelin staining and 3,3′-diaminobenzidine tetrahydrochloride enhanced Turnbull blue staining. Quantification of R2*, myelin and iron intensity were obtained. Variations in R2* were found to be affected differently by myelin and iron content in different regions of multiple sclerosis brains. The data shall inform clinical investigators in addressing the role of T2*/R2* variations as a biomarker of tissue integrity in brains of MS patients, in vivo.
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