Pilot assessment of a human extracellular matrix-based vascular graft in a rabbit model.
Pilot assessment of a human extracellular matrix-based vascular graft in a rabbit model.
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DOI：
10.1016/j.jvs.2016.02.046
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发表时间：
2017-03
影响因子：
4.3
通讯作者：
McFetridge, Peter S.
中科院分区：
文献类型：
--
作者：
Amensag, Salma;Goldberg, Leslie A.;O'Malley, Kerri A.;Rush, Demaretta S.;Berceli, Scott A.;McFetridge, Peter S.
在这里，我们描述了一个小直径的血管移植物构建从卷起的人羊膜（hAM），在体外评价和随后的体内评估其机械和初始生物活性在植入后早期。这种移植物构建方法允许定制移植物尺寸，作为非自体、同种异体、无细胞移植物材料具有广泛的潜在临床适用性。将脱细胞hAM卷成分层导管（3.2-mm直径），其与纤维蛋白结合并冻干。将构建体与人平滑肌细胞（SMC）接种，并在受控的动脉血流动力学条件下培养。SMC显示出在四周的培养期内粘附于支架、在支架内增殖并重塑支架。在培养期结束时，有组织学和生物力学证据表明移植物壁层聚结。将脱细胞hAM导管作为动脉间置移植物手术植入免疫活性兔的颈动脉中。移植物在4周内显示通畅（n=3），无超急性排斥反应或血栓性闭塞。外植体显示出活跃的细胞重塑的组织学证据，移植物壁的内源性细胞再增殖与初始移植物材料的降解同时发生。细胞显示为周向排列，类似于血管中层。显示血管移植物提供支持性支架，允许宿主细胞群体在体内进行细胞浸润和重塑。使用这种方法，可以创建具有指定直径和壁厚的“现成”血管移植物，以满足不同患者群体的特定解剖要求。
Herein we describe a small-diameter vascular graft constructed from rolled human amniotic membrane (hAM), with in vitro evaluation and subsequent in vivo assessment of its mechanical and initial biological viability in the early post-implantation period. This approach for graft construction allows for customization of graft dimensions, with wide-ranging potential clinical applicability as a non-autologous, allogeneic, cell-free graft material. Acellular hAM were rolled into layered conduits (3.2-mm diameter) that were bound with fibrin and lyophilized. Constructs were seeded with human smooth muscle cells (SMC) and cultured under controlled arterial hemodynamic conditions. SMC were shown to adhere to, proliferate within, and remodel the scaffold over a four-week culture period. At the end of the culture period, there was histologic and biomechanical evidence of graft wall layer coalescence. The acellular hAM conduits were surgically implanted as arterial interposition grafts into the carotid arteries of immunocompetent rabbits. Grafts demonstrated patency over four weeks (n=3) with no hyperacute rejection or thrombotic occlusion. Explants displayed histologic evidence of active cellular remodeling, with endogenous cell repopulation of the graft wall concurrent with degradation of initial graft material. Cells were shown to align circumferentially to resemble a vascular medial layer. The vascular grafts were shown to provide a supportive scaffold allowing for cellular infiltration and remodeling by host cell populations in vivo. Using this approach, “off-the-shelf” vascular grafts can be created with specified diameters and wall thicknesses to satisfy specific anatomical requirements in diverse patient populations.
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影响因子：
3.7
作者：
Allen CL;Clare G;Stewart EA;Branch MJ;McIntosh OD;Dadhwal M;Dua HS;Hopkinson A
通讯作者：
Hopkinson A
影响因子：
3.1
作者：
Taggart, David P.
通讯作者：
Taggart, David P.
影响因子：
4.8
作者：
L'Heureux, N;Pâquet, S;Auger, FA
通讯作者：
Auger, FA
影响因子：
4.4
作者：
Hopkinson, Andrew;McIntosh, Richard S.;Dua, Harminder S.
通讯作者：
Dua, Harminder S.
DOI：
10.1016/j.msec.2013.02.016
发表时间：
2013-07-01
影响因子：
7.9
作者：
Karimi, Alireza;Navidbakhsh, Mahdi;Faghihi, Shahab
通讯作者：
Faghihi, Shahab