Coexpression of parental homeologs of leaf dissection related genes are associated with intermediate leaf forms of two allotetraploid yarrows (Achillea, Asteraceae)
Coexpression of parental homeologs of leaf dissection related genes are associated with intermediate leaf forms of two allotetraploid yarrows (Achillea, Asteraceae)
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叶解剖相关基因的亲本同源物的共表达与两种异源四倍体欧蓍草（Achillea，菊科）的中间叶形式相关
DOI：
10.1111/jse.12553
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发表时间：
2020-01
期刊：
影响因子：
--
通讯作者：
Yan-Ping Guo
中科院分区：
文献类型：
--
作者：
Duo Chen;Jin-Xiu Ke;Chu-Ze Shen;Yu-Fen Zhang;Yan-Ping Guo
等位多倍化通常会引起显著的基因组和转录变化，从而促进表型修饰和/或新颖性的发生。四倍体高山菊属菊科植物复合体的遗传变异和亲缘关系已被研究得很清楚。群体遗传学数据表明，它们的祖先为二倍体尖叶紫薇A.acumata(Ledeb.)Sch.毕普。和A.asiatica Serg.，并表明它们保持了两个不同的亲本基因组的相对完整。在表型上，这种复合体在亲本物种之间表现出一系列的叶形变异。为了更好地了解异源四倍体物种在转录水平上的变异来源，我们进行了关于叶片解剖模式的形态和基因表达分析。对四倍体复合体的两个成员--高山紫薇和紫云英进行了叶片性状的形态计量学研究。和它们的二倍体祖先种--尖吻海棠和亚洲海棠。同时，采用逆转录实时定量聚合酶链式反应(RT-qPCR)方法检测了12个叶片解剖相关基因的表达模式。在异源四倍体物种中，重要的叶片解剖相关基因如NAM和PIN在两个二倍体物种之间的中间水平表达。一般来说，在异源四倍体中检测到的大多数基因都在亲本中间水平或接近亲本物种的水平表达。考虑到亲本同源基因对异源四倍体的相对贡献，所分析的基因之间没有一致的模式。总体而言，缺乏叶片解剖相关基因的超前表达可能与异源四倍体高山沙打旺和沙打旺在其亲本物种之间的中间叶表型有关。
Allopolyploidization usually causes prominent genomic and transcriptional changes, and consequently, promotes the occurrence of phenotypic modifications and/or novelties. The tetraploid Achillea alpina L. species complex in the daisy family has been clearly studied concerning genetic variations and parentage. Population genetic data point to their progenitors as the diploid A. acuminata (Ledeb.) Sch. Bip. and A. asiatica Serg., and show that they have kept two distinct parental genomes relatively intact. Phenotypically, this complex displays a range of leaf form variation between the parental species. To better understand the source of variation at the transcriptional level of the allotetraploid species, we undertook morphological and gene expression analyses with regard to leaf dissection patterns. Morphometrics of leaf traits were carried out on two members of the tetraploid complex, A. alpina and A. wilsoniana Heimerl ex Hand.‐Mazz. and their diploid progenitor species, A. acuminata and A. asiatica. In parallel, the expression patterns of a dozen leaf dissection‐related genes were detected using reverse transcription real‐time quantitative polymerase chain reaction (RT‐qPCR) method. In the allotetraploid species, important leaf dissection‐related genes, such as NAM and PIN, were expressed at the intermediate level between the two diploid species. In general, most of the genes examined in the allotetraploids were expressed at mid‐parent levels or close to one of the parental species. Considering the relative contribution of parental homeologs to the allotetraploids, there is no consistent pattern among the genes analyzed. Overall, lack of transgressive expression of leaf dissection‐related genes could be correlated with the intermediate leaf phenotypes of the allotetraploids A. alpina and A. wilsoniana between their parental species.
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影响因子：
10.7
作者：
S. Pophaly;A. Tellier
通讯作者：
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4
作者：
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作者：
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11.6
作者：
Nikovics, Krisztina;Blein, Thomas;Laufs, Patrick
通讯作者：
Laufs, Patrick
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作者：
Thomas, Brian C.;Pedersen, Brent;Freeling, Michael
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