Effect of antimicrobial photodynamic therapy using rose bengal and blue light-emitting diode on Porphyromonas gingivalis in vitro: Influence of oxygen during treatment.
Effect of antimicrobial photodynamic therapy using rose bengal and blue light-emitting diode on Porphyromonas gingivalis in vitro: Influence of oxygen during treatment.
复制标题
使用玫瑰红和蓝色发光二极管进行抗菌光动力疗法对体外牙龈卟啉单胞菌的影响：治疗期间氧气的影响。
作者：
Ayano Uekubo;K. Hiratsuka;A. Aoki;Y. Takeuchi;Y. Abiko;Y. Izumi
目的：玫瑰红（RB）与蓝色LED （BL）的结合已成为抗菌光动力治疗（A - pdt）的新技术形式。本研究旨在探讨氧对RB + BL处理对牙龈卟啉单胞菌体外抗菌效果的影响。材料与方法：P。分别用RB、BL （450 ~ 470 nm; 1 W/cm2, 5 s）或RB + BL在厌氧/好氧条件下处理牙龈细胞。对细胞进行厌氧培养，6-48 h后测定细胞密度（OD600 nm），并在血琼脂板厌氧培养9 d，观察菌落形成情况。在好氧RB + BL处理1 h内检测细菌生长情况，在培养3 h后检测厌氧/好氧RB + BL处理的RNA降解情况。结果：在厌氧条件下，RB + BL在18h后显著抑制细菌生长；48 h后菌落生长和9 d后菌落数与未处理对照相似。厌氧处理组的RNA降解与对照组无显著差异。在好氧条件下，RB + BL立即影响细菌生长并完全抑制生长长达48 h。即使在培养9天后也很少检测到菌落，RNA完全降解。结论：与厌氧处理的抑菌作用不同，好氧RB + BL处理可能通过a- pdt作用具有杀菌作用，导致RNA和细菌细胞在短时间内被破坏。
Aims: A combination of rose bengal (RB) and blue LED (BL) has emerged as a new technical modality for antimicrobial photodynamic therapy (a-PDT). The purpose of this study was to clarify the influence of oxygen on the antimicrobial effect of RB + BL treatment on Porphyromonas gingivalis in vitro.Materials and Methods:P. gingivalis cells were treated with RB, BL (450-470 nm; 1 W/cm2, 5 s), or RB + BL under anaerobic/aerobic conditions. Cells were incubated anaerobically, and the cell density (OD600 nm) was measured after 6-48 h. Additionally, cells were cultured anaerobically on blood agar plates for 9 days, and the resulting colonies were observed. Bacterial growth within 1 h of aerobic RB + BL treatment was examined, and RNA degradation due to anaerobic/aerobic RB + BL treatment was measured after 3 h of culture. Results: Under anaerobic conditions, RB + BL significantly suppressed bacterial growth after 18 h; however, the growth after 48 h and the number of colonies after 9 days were similar to those of the untreated control. RNA degradation in the anaerobic-treatment group was not significantly different from that in the control. Under aerobic conditions, RB + BL immediately affected bacterial growth and completely inhibited growth for up to 48 h. Few colonies were detected even after 9 days of culture, and RNA was completely degraded. Conclusions: Unlike the bacteriostatic effect of anaerobic treatment, aerobic RB + BL treatment may have a bactericidal action via a-PDT effect, resulting in the destruction of RNA and bacterial cells within a short period.
影响因子：
11.3
作者：
Vatansever F;de Melo WC;Avci P;Vecchio D;Sadasivam M;Gupta A;Chandran R;Karimi M;Parizotto NA;Yin R;Tegos GP;Hamblin MR
通讯作者：
Hamblin MR