Effect of short chain fatty acids on the expression of free fatty acid receptor 2 (Ffar2), Ffar3 and early-stage adipogenesis.
Effect of short chain fatty acids on the expression of free fatty acid receptor 2 (Ffar2), Ffar3 and early-stage adipogenesis.
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DOI：
10.1038/nutd.2014.25
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发表时间：
2014-08-04
影响因子：
6.1
通讯作者：
Johnston, J. D.
中科院分区：
文献类型：
--
作者：
Frost, G.;Cai, Z.;Raven, M.;Otway, D. T.;Mushtaq, R.;Johnston, J. D.
脂肪组织对胰岛素敏感性有重要影响。刺激游离脂肪酸受体2 （FFAR2）已被提出影响脂肪细胞分化。我们假设，将前脂肪细胞暴露于短链脂肪酸会诱导协调脂肪形成、甘油三酯积累和瘦素分泌的核受体的早期表达。3T3-L1前脂肪细胞分别在1 μM醋酸酯、0.1-10 μM丙酸或对照物中分化。在实验1中，在分化开始后的48小时内，通过定量PCR检测Ffar2和核受体mRNA的表达。在实验2中，在分化开始后的第0、2、4、6、8和10天，测量细胞外瘦素浓度和细胞内甘油三酯含量。对照细胞表现出与先前报道的基因表达、甘油三酯积累和瘦素分泌相似的时间动态。我们无法检测到ffar3mrna在任何分化阶段的表达。与Ffar2在分化前24小时缺乏表达一致，醋酸盐和丙酸盐对细胞核受体表达没有显著影响。此外，醋酸或丙酸处理没有改变瘦素浓度或甘油三酯含量。总之，通过验证的定量技术，我们观察到丙酸盐或醋酸盐对3T3-L1细胞脂肪形成没有显著影响。
Adipose tissue has a major influence on insulin sensitivity. Stimulation of free fatty acid receptor 2 (FFAR2) has been proposed to influence adipocyte differentiation. We hypothesised that exposing preadipocytes to short chain fatty acids would induce earlier expression of nuclear receptors that co-ordinate adipogenesis, triglyceride accumulation and leptin secretion. 3T3-L1 preadipocytes were differentiated in the presence of 1 μM acetate, 0.1–10 μM propionate or vehicle control. In experiment 1, expression of Ffar2 and nuclear receptor mRNA was measured by quantitative PCR over 48 h following onset of differentiation. In experiment 2, extracellular leptin concentration and intracellular triglyceride content were measured at days 0, 2, 4, 6, 8 and 10 following the onset of differentiation. Control cells exhibited similar temporal dynamics of gene expression, triglyceride accumulation and leptin secretion as reported previously. We were unable to detect expression of Ffar3 mRNA at any stage of differentiation. Consistent with a lack of Ffar2 expression in the first 24 h of differentiation, acetate and propionate had no significant effect on nuclear receptor expression. Furthermore, acetate or propionate treatment did not alter leptin concentration or triglyceride content. In conclusion, we observed no significant effect of propionate or acetate on adipogenesis in 3T3-L1 cells using validated quantitative techniques.
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